Lack of species-specific difference in pulmonary function when using mouse versus human plasma in a mouse model of hemorrhagic shock
Lack of species-specific difference in pulmonary function when using mouse versus human plasma in a mouse model of hemorrhagic shock
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DOI：
10.1097/ta.0000000000001221
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发表时间：
2016-11-01
影响因子：
3.4
通讯作者：
Kozar, Rosemary A.
中科院分区：
文献类型：
--
作者：
Peng, Zhanglong;Pati, Shibani;Kozar, Rosemary A.
背景：临床研究表明，早期和经验性使用血浆可提高失血性休克后的生存率。我们已经在失血性休克的啮齿动物模型中证明，与乳酸林格氏液相比，用血浆复苏对肺具有保护作用。由于我们的长期目标是确定调节血浆在受伤出血患者中保护作用的分子机制，我们在小鼠失血性休克模型中使用了人血浆。当前实验的目的是确定在建立的失血性休克和剖腹手术的小鼠模型中使用人与小鼠血浆时是否对肺损伤有显著的不良影响。小鼠经历剖腹手术和90分钟的失血性休克至35 +/-5 mm Hg的平均动脉压（MAP），然后使用小鼠新鲜冷冻血浆（FFP），人FFP或人冻干血浆。记录休克期间和复苏前30分钟的平均动脉压。3小时后，动物被杀死，和肺收集用于analysis.RESULTS：有一个显着增加，早期MAP时，小鼠FFP复苏动物相比，人FFP或人冻干血浆。然而，尽管存在这些差异，但小鼠肺的分析显示，所有三个血浆组之间的肺组织病理学、肺渗透性或肺水肿无显著差异。中性粒细胞浸润在肺部的分析表明，小鼠FFP减少中性粒细胞流入中性粒细胞染色测量，然而，髓过氧化物酶免疫染色显示没有显着差异，groups.CONCLUSION：研究人血浆在小鼠失血性休克模型是可行的，但没有发现一些差异相比，小鼠血浆为基础的复苏，如MAP复苏后的生理措施。终末器官功能的测量，如肺损伤，在这种急性失血性休克和复苏模型中似乎是相当的。版权所有（C）2016威科医疗集团All rights reserved.
BACKGROUND: Clinical studies have demonstrated that the early and empiric use of plasma improves survival after hemorrhagic shock. We have demonstrated in rodent models of hemorrhagic shock that resuscitation with plasma is protective to the lungs compared with lactated Ringer's solution. As our long-term objective is to determine themolecular mechanisms thatmodulate plasma's protective effects in injured bleeding patients, we have used human plasma in a mouse model of hemorrhagic shock. The goal of the current experiments is to determine if there are significant adverse effects on lung injurywhen using human versus mouse plasma in an establishedmurinemodel of hemorrhagic shock and laparotomy.METHODS: Mice underwent laparotomy and 90 minutes of hemorrhagic shock to a mean arterial pressure (MAP) of 35 +/- 5 mm Hg followed by resuscitation at 1 x shed blood using either mouse fresh frozen plasma (FFP), human FFP, or human lyophilized plasma. Mean arterial pressure was recorded during shock and for the first 30 minutes of resuscitation. After 3 hours, animals were killed, and lungs collected for analysis.RESULTS: There was a significant increase in early MAP when mouse FFP was used to resuscitate animals compared with human FFP or human lyophilized plasma. However, despite these differences, analysis of the mouse lungs revealed no significant differences in pulmonary histopathology, lung permeability, or lung edema between all three plasma groups. Analysis of neutrophil infiltration in the lungs revealed that mouse FFP decreased neutrophil influx as measured by neutrophil staining; however, myeloperoxidase immunostaining revealed no significant differences in between groups.CONCLUSION: The study of human plasma in a mouse model of hemorrhagic shock is feasible but does reveal some differences compared with mouse plasma-based resuscitation in physiologic measures such as MAP postresuscitation. Measures of end organ function such as lung injury appear to be comparable in this acute model of hemorrhagic shock and resuscitation. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved.