Human IL-17: a novel cytokine derived from T cells.
Human IL-17: a novel cytokine derived from T cells.
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DOI：
10.4049/jimmunol.155.12.5483
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发表时间：
1995-12
影响因子：
4.4
通讯作者：
Z. Yao;S. Painter;W. Fanslow;D. Ulrich;B. Macduff;M. Spriggs;R. Armitage
中科院分区：
文献类型：
--
作者：
Z. Yao;S. Painter;W. Fanslow;D. Ulrich;B. Macduff;M. Spriggs;R. Armitage
从CD 4 + T细胞文库中克隆了编码人IL-17（hIL-17）的cDNA。预测的155个氨基酸的序列包含一个N-末端信号肽，并表现出72%的氨基酸同一性与HVS 13，一个开放的阅读框架从T-嗜淋巴细胞疱疹病毒saimiri，和63%与鼠CTLA 8。刺激后，从原代外周血CD 4 + T细胞诱导高水平的hIL-17。重组hIL-17在CV1/EBNA细胞中表达后，以糖基化和非糖基化两种形式分泌。hIL-17.Fc融合蛋白和来自用hIL-17转染的细胞的上清液诱导人成纤维细胞中IL-6和IL-8的产生，并增强细胞内粘附分子-1（ICAM-1）的表面表达。
A cDNA encoding human IL-17 (hIL-17) was cloned from a CD4+ T cell library. The predicted 155-amino acids sequence contains an N-terminal signal peptide and exhibits 72% amino acid identity with HVS13, an open reading frame from a T-lymphotropic Herpesvirus saimiri, and 63% with murine CTLA8. High levels of hIL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation. When expressed in CV1/EBNA cells, recombinant hIL-17 was secreted in both glycosylated and nonglycosylated forms. A hIL-17.Fc fusion protein and supernatants from cells transfected with hIL-17 induced IL-6 and IL-8 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.