Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice.
Comparisons of the biodistribution and toxicological examinations after repeated intravenous administration of silver and gold nanoparticles in mice.
复制标题
纳米银、金纳米粒子在小鼠体内重复静脉给药后的生物分布及毒理学检查比较
DOI：
10.1038/s41598-017-03015-1
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发表时间：
2017-06-12
影响因子：
4.6
通讯作者：
Xu H
中科院分区：
文献类型：
--
作者：
Yang L;Kuang H;Zhang W;Aguilar ZP;Wei H;Xu H
纳米颗粒（NPs）的大小、表面功能化和浓度被认为有助于NPs在体内的分布和毒性结果。然而，NPs的内在化学成分导致其生物分布和毒性分布不一致，这一点很少引起人们的注意。在这项研究中，银纳米粒子（AgNPs）和金纳米粒子（AuNPs）被用来测定小鼠动物的生物分布、毒动力学和遗传毒性差异。结果表明，AgNPs和AuNPs主要沉积在单核吞噬细胞系统（MPS）中，如肝脏和脾脏。特别是，AuNPs似乎主要储存在肝脏，而AgNPs则优先积聚在更多的器官，如心脏、肺、肾等。此外，血液循环和粪便排泄中AgNPs的含量高于AuNPs。小鼠身体和器官质量测量、血液学和生物化学评估以及组织病理学检查表明，在两个月的时间内，AgNPs和AuNPs之间存在轻微的毒性差异。RT-qPCR数据显示，AgNPs诱导了与氧化应激、细胞凋亡和离子转运相关的基因表达的更大变化。我们的观察证明，NPs的化学成分在其体内生物分布和毒性中起着关键作用。
Nanoparticles (NPs) size, surface functionalization, and concentration were claimed to contribute to distribution and toxicity outcomes of NPs in vivo. However, intrinsic chemical compositions of NPs caused inconsistent biodistribution and toxic profiles which attracted little attention. In this study, silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were used to determine the biodistribution, toxickinetic, and genotoxicity variances in murine animals. The results demonstrated AgNPs and AuNPs were primarily deposited in the mononuclear phagocyte system (MPS) such as the liver and spleen. In particular, AuNPs seemed to be prominently stored in the liver, whereas AgNPs preferentially accumulated in more organs such as the heart, lung, kidney, etc. Also, the circulation in the blood and fecal excretions showed higher AgNPs contents in comparison with the AuNPs. Measurements of the mouse body and organ mass, hematology and biochemistry evaluation, and histopathological examinations indicated slight toxic difference between the AgNPs and AuNPs over a period of two months. RT-qPCR data revealed that AgNPs induced greater changes in gene expression with relevance to oxidative stress, apoptosis, and ion transport. Our observations proved that the NPs chemical composition played a critical role in their in vivo biodistribution and toxicity.