Development of acetylchilinesterase during embryogenesis of the ascidian Ciona intestinalis.
Development of acetylchilinesterase during embryogenesis of the ascidian Ciona intestinalis.
复制标题
海鞘胚胎发生过程中乙酰胆碱酯酶的发育。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
J. Whittaker
中科院分区：
文献类型：
--
作者：
T. H. Meedel;J. Whittaker
本文报道了单栖海鞘（Ciona cascidian，L.）选择性酶抑制剂的作用和高浓度底物对酶活性的抑制表明，肌细胞酶是乙酰胆碱酯酶（E. C. 3.1.1.7）。在原肠胚形成后和孵化前，乙酰胆碱酯酶活性增加了35至40倍;孵化后（受精后18小时），这一活动继续增加，在约36小时的发展趋于平稳。组织化学观察表明，孵化前的乙酰胆碱酯酶主要位于肌肉细胞的尾巴，孵化后，它开始在成人的肌肉组织和大脑的细胞。嘌呤霉素和放线菌素D的RNA合成的蛋白质syntnesis的抑制，表明蛋白质和RNA的合成所需的乙酰胆碱酯酶活性的增加，观察未孵化的胚胎。尽管在胚胎发育过程中酶活性的持续增加在所有测试的时间都对嘌呤霉素敏感，但这种增加的放线菌素D敏感性仅限于约11小时发育完成的离散时间。
We have characterized the embryonic muscle cell cholinesterase of the solitary ascidian, Ciona intestinalis (L.). The effects of selective enzyme inhibitors and the inhibition of enzyme activity at high concentrations of substrate suggest that the muscle cell enzyme is an acetylcholinesterase (E.C. 3.1.1.7). After gastrulation and before hatching, acetylcholinesterase activity increased 35- to 40-fold; after hatching (18 hours postfertilization) this activity continued to increase, leveling off at about 36 hours of development. Histochemical observations showed that before hatching acetylcholinesterase was located principally in the muscle cells of the tail and, after hatching, it began to develop in cells of the adult musculature and brain. Inhibition of protein syntnesis by puromycin and of RNA synthesis by actinomycin D, suggest that both protein and RNA synthesis were required for the increase in acetylcholinesterase activity observed in unhatched embryos. Although the continued increase in enzyme activity duirng embryonic development was sensitive to puromycin at all times tested, the actinomycin D sensitivity of this increase was restricted to a discrete time that was completed by about 11 hours of development.