Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
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DOI：
10.1002/9780470151808.sc02d04s10
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发表时间：
2009-09-01
影响因子：
--
通讯作者：
de Vellis, Jean
中科院分区：
文献类型：
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作者：
Espinosa-Jeffrey, Araceli;Wakeman, Dustin R;de Vellis, Jean
在这里，我们文件的生产，分离和维护的少突胶质细胞表型从啮齿动物和人类神经干细胞的协议。我们独特的方法依赖于一系列化学成分确定的培养基，这些培养基针对少突胶质细胞的每个发育阶段进行了专门设计和仔细表征，因为它们从少突胶质细胞祖细胞发展为成熟的髓鞘化少突胶质细胞。
Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.