Lipopolysaccharide-mediated enhancement of zymosan phagocytosis by RAW 264.7 macrophages is independent of opsonins, laminarin, mannan, and complement receptor 3
Lipopolysaccharide-mediated enhancement of zymosan phagocytosis by RAW 264.7 macrophages is independent of opsonins, laminarin, mannan, and complement receptor 3
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DOI：
10.1016/j.jss.2014.03.024
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发表时间：
2014-06-01
影响因子：
2.2
通讯作者：
Sigola, Lynette
中科院分区：
文献类型：
--
作者：
Fuentes, Ana-Lucia;Millis, Leonard;Sigola, Lynette
背景资料：真菌和细菌合并感染在外科环境中很常见;然而，关于多微生物相互作用对先天免疫识别和吞噬作用中涉及的细胞机制的影响知之甚少。材料和方法：酵母聚糖颗粒是酵母酿酒酵母的细胞壁衍生物，用于模拟真菌与宿主免疫细胞的相互作用，因为它们显示碳水化合物，包括β-葡聚糖，是真菌病原体的特征使用体外细胞培养，RAW 264.7巨噬细胞用酵母聚糖攻击，并通过光学显微镜测定吞噬作用。研究了不同浓度脂多糖（LPS）对酵母多糖吞噬功能的影响。此外，转移的上清液从LPS处理的细胞幼稚细胞，可溶性碳水化合物海带多糖，甘露聚糖，或半乳甘露聚糖的影响，以及补体受体3（CR 3）抑制对吞噬功能的影响也determined.Results：LPS增强酵母多糖的吞噬作用在剂量依赖性的方式。将LPS致敏细胞的上清液转移至幼稚细胞对吞噬作用没有影响。海带多糖抑制酵母多糖吞噬幼稚细胞，但不是在LPS引发的细胞。无论是甘露聚糖，半乳甘露聚糖，也没有CR 3抑制有显着的效果摄取unopsonized酵母聚糖在幼稚或LPS处理cells.Conclusions：酵母聚糖识别幼稚细胞抑制海带多糖，但不是甘露聚糖，半乳甘露聚糖，或CR 3抑制。LPS对吞噬作用的增强是海带多糖不敏感的，并且不受上清液因子或酵母聚糖与甘露糖或CR 3受体的结合介导。我们的数据表明，在LPS的存在和不存在的酵母聚糖识别的替代机制。(C)2014爱思唯尔公司All rights reserved.
Background: Fungal and bacterial coinfections are common in surgical settings; however, little is known about the effects of polymicrobial interactions on the cellular mechanisms involved in innate immune recognition and phagocytosis.Materials and methods: Zymosan particles, cell wall derivatives of the yeast Saccharomyces cerevisiae, are used to model fungal interactions with host immune cells since they display carbohydrates, including beta-glucan, that are characteristic of fungal pathogens. Using in vitro cell culture, RAW 264.7 macrophages were challenged with zymosan, and phagocytosis determined via light microscopy. The effects of different concentrations of lipopolysaccharide (LPS) on zymosan phagocytosis were assessed. In addition, the transfer of supernatant from LPS-treated cells to naive cells, the effects of soluble carbohydrates laminarin, mannan, or galactomannan, and the impact of complement receptor 3 (CR3) inhibition on phagocytosis were also determined.Results: LPS enhanced phagocytosis of zymosan in a dose-dependent manner. Transfer of supernatants from LPS-primed cells to naive cells had no effect on phagocytosis. Laminarin inhibited zymosan phagocytosis in naive cells but not in LPS-primed cells. Neither mannan, galactomannan, nor CR3 inhibition had a significant effect on ingestion of unopsonized zymosan in naive or LPS-treated cells.Conclusions: Zymosan recognition by naive cells is inhibited by laminarin, but not mannan, galactomannan, or CR3 inhibition. LPS enhancement of phagocytosis is laminarin insensitive and not mediated by supernatant factors or zymosan engagement by the mannose or CR3 receptors. Our data suggest alternative mechanisms of zymosan recognition in the presence and absence of LPS. (C) 2014 Elsevier Inc. All rights reserved.