Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742
Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742
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DOI：
10.5152/tjg.2019.18451
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发表时间：
2019-09-01
影响因子：
1.2
通讯作者：
Moazamian, Elham
中科院分区：
文献类型：
--
作者：
Bahmani, Sepideh;Azarpira, Negar;Moazamian, Elham
背景/目的：用作益生菌的菌种是乳酸菌。它们中的大多数属于双歧杆菌属和乳杆菌属。本研究的目的是分离和鉴定双歧杆菌，并评价其24 h和120 h的无细胞上清液（CFS）从两种培养物对结肠癌细胞株的影响。材料和方法：在本研究中，收集了84个样品的乳制品，婴儿粪便，益生菌胶囊，双歧杆菌分离。采用革兰氏染色、生化试验和分子鉴定等方法对双歧杆菌进行分离和鉴定。结果：在本研究中，共鉴定出17株双歧杆菌。结果表明，双歧杆菌最常见的婴儿粪便和乳制品，而最低的比例与当地牛奶。在CFS对结肠癌细胞株的作用后，从婴儿粪便和益生菌胶囊中鉴定出两个分离物，它们对癌细胞生长的抑制能力最高。结论：研究表明，经常摄入益生菌可以预防结直肠癌的发生。在本研究中，所产生的CFS可以抑制结肠癌细胞的生长。总之，益生菌作为结肠癌治疗的一种新方法具有良好的潜力。
Background/Aims: Bacteria species, which are used as probiotics, are lactic acid bacteria. The majority of them are under the genera Bifidobacterium and Lactobacillus. The aim of the present study was to isolate and identify Bifidobacterium and to evaluate the effects of their 24 h and 120 h cell-free supernatants (CFS) from both cultures on colon cancer cell line.Materials and Methods: In the present study, 84 samples of dairy products, infant feces, and probiotic capsule were collected, and Bifidobacterium was isolated. Gram stain, biochemical tests, and molecular identification were done for the isolation and identification of Bifidobacterium. Cytotoxicity effects of CFS derived from both cultures of isolated Bifidobacterium were assessed on colon cancer cell lines.Results: In the present study, 17 isolates of Bifidobacterium were identified. The results show that Bifidobacterium was most frequently associated with infant feces and dairy products, whereas the lowest rate was associated with local milk. After the effects of CFS on colon cancer cell line, two isolates were identified from infant feces and probiotic capsule; they had the highest ability in inhibiting the growth of cancer cells. Bifidobacterium bifidum was effective in combating cancer cells and was associated with a substantial improvement in gastrointestinal cancer.Conclusion: The study has shown that the regular ingested probiotics could prevent the development of colorectal cancer. During the present study, the produced CFS could inhibit the growth of colon cancer cells. In conclusion, probiotics have good potential to be introduced as a new approach to colon cancer treatment.