Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity
Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity
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稻根响应镉毒性的蛋白质复合物和蛋白质组谱
DOI：
10.1007/s11738-015-1936-2
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发表时间：
2015-09-01
影响因子：
2.6
通讯作者：
He, Huaqin
中科院分区：
文献类型：
--
作者：
Lin, Shoukai;Wan, Weifeng;He, Huaqin
镉（Cd）是一种非必需的有毒重金属元素，对水稻生长、食品安全乃至公众健康危害极大。以耐Cd胁迫的汕优63和敏感Cd胁迫的日本bare两个水稻品种为材料，研究了不同阶段Cd胁迫对水稻蛋白表达和蛋白-蛋白相互作用的影响。结果表明，2个品种的根系均有Cd积累，但汕优63的根系Cd含量显著低于日本。8种蛋白在2个水稻品种中均上调表达，但在汕优63中的表达丰度高于在日本裸中的表达丰度，表明这8种蛋白的相对丰度与水稻品种抵御Cd胁迫的能力呈正相关。根据Gene Ontology注释，这8个蛋白中有4个参与能量代谢，4个参与胁迫耐受。结果表明，4种GSTs、1种半胱氨酸合成酶和1种蛋白质二硫异构酶在Cd胁迫下形成了稳定的蛋白质复合物，而该蛋白复合物在Cd胁迫下不出现在日本根中。由于具有相同的结构域，4种谷胱甘肽s -转移酶（GSTs）和半胱氨酸合成酶有可能相互作用并组成一个蛋白质复合物来解毒汕优63水稻植株的Cd。我们的研究结果为水稻对Cd胁迫的解毒机制提供了新的认识，并可能为今后的研究提供参考。
Cadmium (Cd), a non-essential and toxic heavy metal element, is extremely harmful for rice growth, food safety and even public health. In this paper, two rice varieties, Shanyou 63 (Tolerant to Cd stress) and Nipponbare (Susceptible to Cd stress), were employed as materials to investigate the protein expression and protein–protein interaction in rice in response to different stages of Cd stress. The result showed that Cd accumulated in the root of both rice cultivars, but the Cd content in the root of Shanyou 63 was significantly lower than that in Nipponbare. Eight proteins were up-regulated in the two rice cultivars, but the expressing abundance of these eight proteins in Shanyou 63 was statistically higher than that in Nipponbare, indicating that the relative abundance of these eight proteins was positively related to the ability of rice cultivars to avoid Cd stress. According to Gene Ontology annotation, four of these eight proteins were involved in energy metabolism, while four participated in stress tolerance. Native-PAGE and LC–MS/MS methods revealed that four GSTs, one cysteine synthase and one protein disulfide isomerase formed a stable protein complex in Shanyou 63 root in response to 3-, 5- and 7-day Cd stresses, but this protein complex did not appear in Nipponbare root under Cd stress. Owing to the same domain, the four glutathione S-transferases (GSTs) and cysteine synthase had the potential to interact and compose a protein complex to detoxify Cd in Shanyou 63 rice plants. Our results provide new insight into the detoxification mechanism of rice plant in response to Cd stress and may prove to be useful for the future research.