Comparative Studies of Actin- and Rho-Specific ADP-Ribosylating Toxins: Insight from Structural Biology
Comparative Studies of Actin- and Rho-Specific ADP-Ribosylating Toxins: Insight from Structural Biology
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肌动蛋白和 Rho 特异性 ADP 核糖基化毒素的比较研究：来自结构生物学的见解
DOI：
10.1007/82_2016_23
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yoshida T.
中科院分区：
文献类型：
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作者：
Tsuge H;Tsurumura T;Toda A;Murata H;Toniti W;Yoshida T.
单ADP-核糖基化是细菌毒素进行的主要翻译后修饰，其将ADP-核糖部分转移到底物受体残基。肌动蛋白和Rho特异性ADP核糖基化毒素（ART）是已知具有非常相似的三级结构但完全不同的靶点的典型ART。肌动蛋白特异性ART是二元毒素的A组分，ADP-核糖基化肌动蛋白Arg 177，导致肌动蛋白细胞骨架的解聚。另一方面，C3样外切酶是Rho特异性ART，在Asn 41处的ADP-核糖基化Rho GTP酶，对肌动蛋白细胞骨架产生间接影响。本文综述了肌动蛋白和Rho特异性ART的异同，特别是在其底物识别和细胞进入机制方面，基于结构研究。
Mono-ADP-ribosylation is a major post-translational modification performed by bacterial toxins, which transfer an ADP-ribose moiety to a substrate acceptor residue. Actin- and Rho-specific ADP-ribosylating toxins (ARTs) are typical ARTs known to have very similar tertiary structures but totally different targets. Actin-specific ARTs are the A components of binary toxins, ADP-ribosylate actin at Arg177, leading to the depolymerization of the actin cytoskeleton. On the other hand, C3-like exoenzymes are Rho-specific ARTs, ADP-ribosylate Rho GTPases at Asn41, exerting an indirect effect on the actin cytoskeleton. This review focuses on the differences and similarities of actin- and Rho-specific ARTs, especially with respect to their substrate recognition and cell entry mechanisms, based on structural studies.