Seasonal variability of bacteria in fine and coarse urban air particulate matter
Seasonal variability of bacteria in fine and coarse urban air particulate matter
复制标题
DOI：
10.1007/s00253-010-3048-7
复制
发表时间：
2011-04-01
影响因子：
5
通讯作者：
Bestetti, Giuseppina
中科院分区：
文献类型：
--
作者：
Franzetti, Andrea;Gandolfi, Isabella;Bestetti, Giuseppina
目前的知识与空气中的颗粒物，特别是在城市地区的微生物群落是有限的。本研究旨在通过焦磷酸测序技术描述与粗颗粒物（PM10）和细颗粒物（PM2.5）相关的微生物群落来填补这一空白。在米兰（意大利）夏季3个月和冬季3个月内，在Teflon过滤器上对颗粒物进行采样，并从过滤器提取的DNA中扩增基因16 S rRNA的高变V3区。结果表明，微生物群落的季节变化很大，夏季以植物相关细菌为主，冬季以孢子形成细菌为主。PM10和PM2.5中的细菌群落也因季节而异。在所有的样品中，一个高的物种丰富度，与土壤相媲美，但均匀度较低的被发现的结果表明，不仅可以颗粒物的来源影响特定的细菌群的存在，但也可以塑造环境因素和压力的细菌群落。
The current knowledge about the microbial communities associated with airborne particulate matter, particularly in urban areas, is limited. This study aims to fill this gap by describing the microbial community associated with coarse (PM10) and fine (PM2.5) particulate matter using pyrosequencing. Particulate matter was sampled on Teflon filters over 3 months in summer and 3 months in winter in Milan (Italy), and the hypervariable V3 region of the gene 16S rRNA amplified from the DNA extracted from the filters. The results showed large seasonal variations in the microbial communities, with plant-associated bacteria dominating in summer and spore-forming bacteria in winter. Bacterial communities from PM10 and PM2.5 were also found to differ from each other by season. In all samples, a high species richness, comparable with that of soils, but a low evenness was found. The results suggest that not only can the sources of the particulate influence the presence of specific bacterial groups but also that environmental factors and stresses can shape the bacterial community.