Synergistic recognition of an epigenetic DNA element by pleiohomeotic and a Polycomb core complex
Synergistic recognition of an epigenetic DNA element by pleiohomeotic and a Polycomb core complex
复制标题
DOI：
10.1101/gad.347005
复制
发表时间：
2005-08-01
影响因子：
10.5
通讯作者：
Verrijzer, CP
中科院分区：
文献类型：
--
作者：
Mohd-Sarip, A;Cléard, F;Verrijzer, CP
多梳应答元件（Polycomb response elements，PRE）是一种顺式作用的DNA元件，通过多梳组蛋白（Polycomb group，PcG）介导表观遗传基因沉默。在这里，我们报告，Pleiohomeotic（PHO）和多蛋白Polycomb核心复合物（PCC）的高度合作绑定到PRE。我们确定了一个保守的序列基序，命名为PCC结合元件（PBE），这是所需的PcG沉默在体内。PHO位点和PBE作为一个整合的DNA平台，用于协同组装抑制性PHO/PCC复合物。我们称这种核蛋白复合物为沉默体，以反映其组装基础的分子原理与形成增强体的分子原理惊人地相似。
Polycomb response elements (PREs) are cis-acting DNA elements that mediate epigenetic gene silencing by Polycomb group (PcG) proteins. Here, we report that Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to PREs. We identified a conserved sequence motif, named PCC-binding element (PBE), which is required for PcG silencing in vivo. PHO sites and PBEs function as an integrated DNA platform for the synergistic assembly of a repressive PHO/PCC complex. We termed this nucleoprotein complex silenceosome to reflect that the molecular principles underpinning its assemblage are surprisingly similar to those that make an enhanceosome.