A nationwide study on Borrelia burgdorferi s.l. infection rates in questing Ixodes ricinus: a six-year snapshot study in protected recreational areas in England and Wales
A nationwide study on Borrelia burgdorferi s.l. infection rates in questing Ixodes ricinus: a six-year snapshot study in protected recreational areas in England and Wales
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DOI：
10.1111/mve.12503
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发表时间：
2021-01-07
影响因子：
1.9
通讯作者：
Medlock, J. M.
中科院分区：
文献类型：
--
作者：
Cull, B.;Hansford, K. M.;Medlock, J. M.
了解伯氏疏螺旋体的变异。对评估莱姆型疏螺旋体病传播的可能性而言，软体硬蜱的感染率是重要的。本研究旨在调查伯氏杆菌的感染率。在英格兰和威尔士的24个野外地点，重点关注国家公园(NPs)和杰出自然美景地区(AONBs)受保护的娱乐区，以提供多年来的可比数据，以评估B.burgdorferi s.l的时空变化。感染。从2014年到2019年，每年春天都会与公园护林员合作收集探寻扁虱。用PAN-疏螺旋体定量聚合酶链式反应和米氏疏螺旋体特异的定量聚合酶链式反应对4104头若虫进行了分析，并进行了测序以确定伯氏疏螺旋体。基因种。站点特有的B.burgdorferi s.l.蓖麻若虫感染率为0%~24%，历年总感染率为2.5%~5.1%。测序样品的基因组组成分别为62.5%的加里氏杆菌、20.3%的瓦氏杆菌和17.2%的阿氏杆菌。在0.2%的硬蜱中检出宫本疏螺旋体。这项研究增加了我们对伯氏杆菌的认识。在英格兰和威尔士，公众用于户外活动的地区发生了感染，突显了空间和时间的变异性，这可能会影响受感染的扁虱叮咬对人类不断变化的风险。
Understanding the variation in Borrelia burgdorferi s.l. infection rates in Ixodes ricinus ticks is important for assessing the potential for Lyme borreliosis transmission. This study aimed to investigate infection rates of B. burgdorferi s.l. bacteria in I. ricinus across 24 field sites in England and Wales, focussing on protected recreational areas in National Parks (NPs) and Areas of Outstanding Natural Beauty (AONBs), to provide comparable data across multiple years to assess spatio-temporal changes in B. burgdorferi s.l. infection. Working with park rangers, questing ticks were collected each spring from 2014 to 2019. A subset of ticks, 4104 nymphs, were analysed using a pan-Borrelia qPCR assay, as well as a Borrelia miyamotoi-specific qPCR, and sequenced to determine Borrelia burgdorferi s.l. genospecies. Site-specific B. burgdorferi s.l. infection rates in I. ricinus nymphs varied from 0% to 24%, with overall infection rates ranging from 2.5% to 5.1% across the years. Genospecies composition of sequenced samples was 62.5% B. garinii, 20.3% B. valaisiana and 17.2% B. afzelii. Borrelia miyamotoi was detected in 0.2% of ticks. This study increases our knowledge on B. burgdorferi s.l.. infection in areas used by the public for outdoor activity across England and Wales, highlighting the spatial and temporal variability which can impact the changing risk to humans from infected tick bites.