Genome-wide analysis of light-inducible responses reveals hierarchical light signalling in Neurospora
Genome-wide analysis of light-inducible responses reveals hierarchical light signalling in Neurospora
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DOI：
10.1038/emboj.2009.54
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发表时间：
2009-04-22
期刊：
影响因子：
11.4
通讯作者：
Loros, Jennifer J.
中科院分区：
文献类型：
--
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
白色领-1（WC-1）和白色领-2（WC-2）是粗糙脉孢菌光介导的反应所必需的，但基因诱导的分子机制和其他真实的和假定的光感受器的作用仍然很差的特点。全基因组微阵列的无监督分层聚类揭示了5.6%的可检测转录本，包括几种新的介质，它们是早期或晚期光响应的。证据表明，在没有占主导地位的光感受，这里证实，白色衣领复杂（WCC），调节这两种类型的光反应。VVD主要调节迟发反应，而光响应性淹没原腹膜炎-1（p-1），一个加塔家族转录因子，是必不可少的最迟的光基因表达。在15分钟的光刺激后，WCC直接结合sub-1启动子。生物信息学分析检测许多早期光响应元件（ELRE），以及鉴定晚期光响应启动子的野生型活性所需的晚期光响应元件（LLRE）。这些数据提供了一个全球性的图片转录响应光，以及照明的顺式和反式作用元件，包括管理的光生物反应的调节信号级联。
White collar-1 (WC-1) and white collar-2 (WC-2) are essential for light-mediated responses in Neurospora crassa, but the molecular mechanisms underlying gene induction and the roles of other real and putative photoreceptors remain poorly characterized. Unsupervised hierarchical clustering of genome-wide microarrays reveals 5.6% of detectable transcripts, including several novel mediators, that are either early or late light responsive. Evidence is shown for photoreception in the absence of the dominant, and here confirmed, white collar complex (WCC) that regulates both types of light responses. VVD primarily modulates late responses, whereas light-responsive submerged protoperithecia-1 (SUB-1), a GATA family transcription factor, is essential for most late light gene expression. After a 15-min light stimulus, the WCC directly binds the sub-1 promoter. Bioinformatics analysis detects many early light response elements (ELREs), as well as identifying a late light response element (LLRE) required for wild-type activity of late light response promoters. The data provide a global picture of transcriptional response to light, as well as illuminating the cis- and trans-acting elements comprising the regulatory signalling cascade that governs the photobiological response.