Nanoparticles with dextran/chitosan shell and BSA/chitosan core-Doxorubicin loading and delivery
Nanoparticles with dextran/chitosan shell and BSA/chitosan core-Doxorubicin loading and delivery
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具有葡聚糖/壳聚糖壳和 BSA/壳聚糖核的纳米颗粒-阿霉素的负载和递送
DOI：
10.1016/j.ijpharm.2010.03.063
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发表时间：
2010-06-30
影响因子：
5.8
通讯作者：
Huang, Chong
中科院分区：
文献类型：
--
作者：
Qi, Jianing;Yao, Ping;Huang, Chong
利用牛血清白蛋白（BSA）与壳聚糖之间的静电吸引作用和BSA的凝胶化作用，将BSA-葡聚糖-壳聚糖复合物与壳聚糖混合加热，制备了生物相容性的BSA-葡聚糖-壳聚糖纳米粒。通过Maillard反应制备BSA-葡聚糖缀合物。通过光散射、zeta电位、原子力显微镜和芘荧光等手段对纳米粒子进行了表征。具有球形形状和130-230 nm的流体动力学直径的纳米颗粒在生理条件下是稳定的。通过纳米颗粒与阿霉素之间的静电和疏水相互作用，将其混合物的pH值改变为7.4后，阿霉素可以有效地负载到纳米颗粒中。通过小鼠腹水型肝癌H22荷瘤小鼠的抑瘤率和生存率研究阿霉素纳米粒的抗肿瘤作用。载药纳米粒能显著降低阿霉素的毒性，显著提高荷瘤小鼠的生存率。(c)2010爱思唯尔有限公司版权所有。
Biocompatible bovine serum albumin (BSA)-dextran-chitosan nanoparticles were fabricated by heating the mixture of chitosan and BSA-dextran conjugates by virtue of the electrostatic attraction between BSA and chitosan as well as the gelation of BSA. The BSA-dextran conjugates were prepared by Maillard reaction. The nanoparticles were characterized by light scattering, zeta-potential, atomic force microscopy and pyrene fluorescence. The nanoparticles having a spherical shape and hydrodynamic diameters of 130-230 nm are stable in physiological condition. Doxorubicin can be effectively loaded into the nanoparticles after changing the pH of their mixture to 7.4 by virtue of the electrostatic and hydrophobic interactions between the nanoparticles and doxorubicin. The antitumor effects of doxorubicin loaded nanoparticles were investigated by the tumor inhibition and survivability of murine ascites hepatoma H22 tumor-bearing mice. The loaded nanoparticles can largely decrease the toxicity of doxorubicin and significantly increase the survivability of the tumor-bearing mice. (c) 2010 Elsevier B.V. All rights reserved.