Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma.
Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma.
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DOI：
10.3892/ol.2016.4362
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发表时间：
2016-05
期刊：
影响因子：
2.9
通讯作者：
Huang MZ
中科院分区：
文献类型：
--
作者：
Li DQ;Qiu M;Nie XM;Gui R;Huang MZ
肝癌发生是一个多基因改变的逐步过程。了解诱导肝癌发生的分子机制可能有助于提高肝细胞癌（HCC）患者的筛查、预防和治疗。近年来，含氧化硝基结构域蛋白1（NOR1）基因在体外实验中被证实在肝癌的发生发展中起重要作用。本研究旨在检测正常肝脏、肝炎、肝硬化和HCC组织中NOR1 mRNA和蛋白的表达，共同代表HCC的发展过程。同时分析NOR1表达与肝癌患者临床病理参数的关系。分别采用原位杂交和免疫组织化学方法检测正常肝、肝炎、肝硬化和肝癌组织中NOR1 mRNA和蛋白的表达。结果表明，肝炎、肝硬化组织中NOR1蛋白和mRNA的阳性表达率与正常肝组织无显著性差异。但与正常肝组织相比，肝癌组织中NOR1蛋白和mRNA表达的阳性率明显增高。此外，病理分化程度低、TNM分期高的HCC患者中，NOR1蛋白表达阳性率较高。总之，本研究首次提供了证据，在人类肝癌组织中的NOR1的表达增加，其与病理分期和TNM状态的相关性。这些结果表明，NOR1可能参与了HCC的进展，并可作为HCC发展的预测生物标志物。
Hepatocarcinogenesis is a stepwise process during which multiple genes are altered. Understanding the molecular mechanisms that induce hepatocarcinogenesis may improve the screening, prevention and treatment of patients with hepatocellular carcinoma (HCC). In recent years, the oxidored-nitro domain-containing protein 1 (NOR1) gene has been identified to have an important role in the development of HCC in vitro experiments. The current study aimed to examine the expression of NOR1 mRNA and protein expression in specimens of normal liver, hepatitis, cirrhosis and HCC, together representing the process of HCC development. Furthermore, the association between NOR1 expression and clinicopathological parameters of HCC patients was analyzed. Tissue microarrays containing the specimens of human normal liver, hepatitis, cirrhosis and HCC were purchased, and in situ hybridization and immunohistochemistry were used to detect the expression of NOR1 mRNA and protein expression, respectively. It was revealed that the positive rate of NOR1 protein and mRNA expression in the specimens of hepatitis and cirrhosis were not significantly different from that in the normal liver samples. However, the specimens of HCC exhibited an increased positive rate of NOR1 protein and mRNA expression in comparison with the normal liver samples. In addition, a higher positive rate of NOR1 protein expression was observed in HCC patients with a poor pathological differentiation grade and high tumor node metastasis (TNM) stage. In conclusion, the present study provides evidence, for the first time, of the increased expression of NOR1 in human HCC tissues, and its correlation with the pathological stage and TNM status. These findings indicate that NOR1 may be involved in the progression of HCC and it could be employed as a predictive biomarker in HCC development.