The extended survival of tw5/tw5 mouse embryo cells in vitro.
The extended survival of tw5/tw5 mouse embryo cells in vitro.
复制标题
tw5/tw5 小鼠胚胎细胞体外存活时间延长。
DOI：
10.1017/s0016672300025155
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Hillman,N
中科院分区：
文献类型：
--
作者：
Garside,W;Hillman,N
tw 5单倍型是一种隐性突变，当在小鼠胚胎中植入后纯合时是致命的。进行这一系列研究以确定tw 5/tw 5基因型对体外胚胎发育的影响。比较了+ /tw 5自交系的囊胚胚胎与T/ +和+ / +雌性和+ /tw 5雄性交配获得的对照囊胚在两种培养基中体外继续发育的能力。数据表明，在任一培养基中，在任何一天培养至相当于妊娠第21天，实验和对照囊胚胚胎的贴壁和生长或含有内细胞团的百分比之间无显著差异。这些结果表明，体外培养可显著延长tw 5/tw 5胚胎细胞的寿命。
The tw5 haplotype is a recessive mutation which is lethal when homozygous in mouse embryos following implantation. This series of studies was undertaken to determine the effect of the tw5/tw5 genotype on embryos developing in vitro. Blastocyst embryos from + / tw5inter se matings were compared with control blastocysts obtained from matings between T/ + and + / + females and + / tw5 males for their abilities to continue development in vitro in two culture media. The data show that there are no significant differences between the percentages of experimental and control blastocyst embryos which attach and outgrow or which contain inner cell masses on any day of culture up to equivalent gestation day 21 in either media. These findings show that the life span of cells from tw5 / tw5 embryos can be extended significantly by in vitro culture.