ULTRASTRUCTURE OF VEILLONELLA + MORPHOLOGICAL CORRELATION OF OUTER MEMBRANE WITH PARTICLES ASSOCIATED WITH ENDOTOXIC ACTIVITY
ULTRASTRUCTURE OF VEILLONELLA + MORPHOLOGICAL CORRELATION OF OUTER MEMBRANE WITH PARTICLES ASSOCIATED WITH ENDOTOXIC ACTIVITY
复制标题
DOI：
10.1128/jb.88.5.1482-1492.1964
复制
发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
MERGENHAGEN, SE
中科院分区：
文献类型：
--
作者：
BLADEN, HA;MERGENHAGEN, SE
将正常的、经苯酚水提取和溶菌酶处理的细孔菌细胞包埋于Vestopal-W中，切片并电镜观察。采用正、阴性对照法对正常细胞及酚水提取物（内毒素）进行检测。在正常细胞的薄切片中，在原生质周围观察到三个独立的结构实体，它们被称为外膜、实膜和质膜。外膜是由两层致密层（30a）和一层较低密度层（20a）组成的膜，并沿着一个卷曲的连续路径环绕细胞。固体膜呈紧绷、致密的结构，宽度为100 - 500a，与外膜之间的距离高达几百埃。质膜为单元型膜。经苯酚-水处理后，细胞外膜缺失，但细胞因有固体膜而保持完整。对酚水提取物（内毒素）的观察显示，主要是圆形颗粒或圆盘，其高度与外膜的宽度大致相同。阴性染色的全细胞表面结构相似。溶菌酶对细胞外膜无影响；但实膜呈弥散状，常消失，提示外膜与实膜是分离的结构。
Normal", phenol-water extracted and lysozyme-treated Veillonella cells were embedded in Vestopal-W, sectioned and examined by electron microscopy. Normal cells as well as the phenol-water extract (endotoxin) were examined by negative and positive contrast technic. In thin sections of normal cells, three separate structural entities were observed surrounding the protoplasm, and were referred to as the outer membrane, the solid membrane and the plasma membrane. The outer membrane was a membrane composed of two dense layers (30 A) separated by a less-dense layer (20 A), and followed a convoluted and continuous path around the cell. The solid membrane appeared as a taut, dense structure 100 to 500 A wide, and was separated from the outer membrane by up to several hundred Angstroms. The plasma membrane was a unit-type membrane. After cells were treated with phenol-water, the outer membrane was absent, but the cells remained intact owing to the solid membrane. Observation of the phenol-water extract (endotoxin) revealed predominantly circular particles or discs which had approximately the same dimensions in height as the outer membrane had in width. Negatively stained whole cells showed similar structures on their surface. Lysozyme treatment of the cells did not affect the outer membrane; however, the solid membrane became diffuse and often disappeared, suggesting that the outer membrane and the solid membrane were separate structures.