Clear-cut photo-regulation of the formation and dissociation of the DNA duplex by modified oligonucleotide involving multiple azobenzenes.
Clear-cut photo-regulation of the formation and dissociation of the DNA duplex by modified oligonucleotide involving multiple azobenzenes.
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DOI：
10.1093/nass/49.1.35
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Komiyama, Makoto
中科院分区：
文献类型：
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作者：
Asanuma, Hiroyuki;Matsunaga, Daijiro;Komiyama, Makoto
在D-苏氨酸上的寡核苷酸中引入多个偶氮苯，以清晰地光调节DNA双链的形成和解离。当偶氮苯以反式形式存在时，引入多个偶氮苯(偶氮苯：碱基=1：2)与其天然双链相比根本不干扰双链的形成。相反，当偶氮苯以顺式形式存在时，熔融温度(Tm)随着偶氮苯数量的增加而均匀降低。结果表明，在生理条件下，双链的形成得到了清晰的光调控。此外，还获得了这些偶氮苯系留寡核苷酸的最近邻参数。结果表明，由这些参数估算的TMS与实测值吻合较好。
Multiple azobenzenes were introduced into the oligonucleotide on D-threoninol for the clear-cut photo-regulation of the formation and dissociation of DNA duplex. When azobenzenes took trans form, introduction of multiple azobenzenes (azobenzenes:nucleobases = 1:2) did not interfere with duplex formation at all compared with their native duplex. In contrast, melting temperature (Tm) uniformly decreased with the number of azobenzenes when they took cis-form. As a result, clear-cut photo-regulation of the duplex formation was attained under physiological conditions. Furthermore, nearest-neighbor parameters for these azobenzene-tethered oligonucleotides were obtained. It was found that Tms estimated from these parameters well coincided with those of measured ones.