Pesticides and Essential Minerals Modify Endogenous Antioxidants and Cytochrome P450 in Tissues of Rats
Pesticides and Essential Minerals Modify Endogenous Antioxidants and Cytochrome P450 in Tissues of Rats
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DOI：
10.1080/03601230500189709
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
F. Bebe;M. Panemangalore
中科院分区：
文献类型：
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作者：
F. Bebe;M. Panemangalore
在雄性Sprague道利（SD）大鼠（175-200 g）中进行了两项实验，以确定血液、肝脏和小肠粘膜（IM）中内源性抗氧化剂超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、细胞色素P450（乙氧基试卤灵脱乙基酶; EROD）活性和谷胱甘肽（GSH）浓度的变化。在两个实验中，六只大鼠/组被喂食基于AIN-93 M饮食（对照）或相同的饮食，所述饮食经修改以含有以下组合的500 mg钙（低Ca）、7 mg Zn（低Zn）：2 mg铜（低Cu）、60 mg锌（高Zn）或12 mg铜（高Cu）：对照组、LCa/LZn、LCa/LZn/LCu或HZn/HCu，含或不含25%LD 50的乙酰甲胺磷、硫丹和福美双农药混合物，持续4或2周。杀虫剂使所有组的摄食量和体重增加减少了28%。HZn/HCu组、LCa/LZn/LCu组和HZn/HCu组红细胞SOD活性均高于对照组（P# 0.05）。与对照组相比，在有和没有农药的所有组中，血浆GPX下降超过55%。LCa/LZn/LCu和HZn/HCu饮食与不农药减少GPX在IM高达88%，40%，和74%，分别比对照。血浆谷胱甘肽比对照组高出约20%，在大多数组的饮食中有和没有农药。HZn/HCu组的肝脏和IM GSH高于对照组，而LCa/LZn和LCa/LZn/LCu组的IM GSH浓度低于对照组（P#0.05）。所有三个实验饮食有和没有农药对肝脏EROD活性有显着影响（P #0.05）。结果表明，内源性抗氧化剂和EROD的独立修改的饮食锌和铜水平和农药。
Two experiments were conducted in male Sprague Dawley (SD) rats (175–200 g) to determine changes in the activities of endogenous antioxidants superoxide dismutase (SOD), glutathione peroxidase (GPX), cytochrome P450 (ethoxyresorufin deethylase; EROD) and concentrations of glutathione (GSH) in the blood, liver, and small intestinal mucosa (IM). In both experiments, six rats/group were fed diets based on the AIN-93M diet (Control) or the same modified to contain either 500 mg calcium (Low Ca), 7 mg Zn (Low Zn): 2 mg copper (Low Cu), 60 mg zinc (High Zn) or 12 mg copper (High Cu) in the following combination: Control, LCa/LZn, LCa/LZn/LCu, or HZn/HCu, with and without a pesticide mixture containing acephate, endosulfan, and thiram at 25% LD50 for four or two weeks. Pesticides decreased feed intake and weight gain in all groups by 28%. Erythrocyte SOD was higher than control in the HZn/HCu group and in the LCa/LZn/LCu and HZn/HCu groups with pesticide (P# 0.05). Plasma GPX declined by more than 55% in all the groups with and without pesticides compared to the control. The LCa/LZn/LCu and HZn/HCu diets with and without pesticides reduced GPX in the IM by up to 88%, 40%, and 74%, respectively, than the control. Plasma GSH was about 20% higher than the control in most groups with and without pesticides in the diet. Liver and IM GSH were higher than the control in the HZn/HCu group, whereas IM GSH concentrations were lower than the control in the LCa/LZn and LCa/LZn/LCu groups (P#0.05). All three experimental diets with and without pesticides had a significant effect on liver EROD activity (P#0.05). The results indicate that endogenous antioxidants and EROD were independently modified by dietary zinc and copper levels and pesticides.