JNK signaling pathway required for wound healing in regenerating Drosophila wing imaginal discs
JNK signaling pathway required for wound healing in regenerating Drosophila wing imaginal discs
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DOI：
10.1016/j.ydbio.2005.01.002
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发表时间：
2005-04-01
影响因子：
2.7
通讯作者：
Baguñà, J
中科院分区：
文献类型：
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作者：
Bosch, M;Serras, F;Baguñà, J
我们已经研究了伤口愈合过程中的果蝇翅成虫盘碎片再生共聚焦显微镜和评估JNK途径的组成部分在这个过程中的作用。切割后，伤口边缘的柱状和周足上皮细胞开始通过肌动蛋白索的形成和收缩闭合伤口。随后是通过来自两个上皮的丝状伪足突起的拉链过程，从椎间盘的近端到远端区域编织伤口边缘。JNK通路的激活参与了这一过程。在伤口边缘的几排细胞中诱导了皱褶（puc）表达，而由半翅目、篮状和Dfos突变体引起的JNK途径活性的缺失损害了伤口愈合。这些缺陷伴随着puc表达的降低或丧失。为了支持puc在伤口愈合中的作用，通过降低puc功能来挽救hep突变表型，而puc的过表达抑制伤口愈合。总之，这些结果证明了JNK通路在成虫椎间盘伤口愈合中的作用，类似于其他愈合过程，如果蝇胚胎背侧闭合，胸部闭合和成年上皮伤口愈合的报道。还强调了与这些过程的差异。(c)2005年爱思唯尔公司All rights reserved.
We have examined wound healing during regeneration of Drosophila wing imaginal discs fragments by confocal microscopy and assessed the role of components of the JNK pathway in this process. After cutting, columnar and peripodial epithelia cells at the wound edge start to close the wound through formation and contraction of an actin cable. This is followed by a zipping process through filopodial protrusions from both epithelia knitting the wound edges from proximal to distal areas of the disc. Activation of the JNK pathway is involved in such process. puckered (puc) expression is induced in several rows of cells at the edge of the wound, whereas absence of JNK pathway activity brought about by hemipterous, basket, and Dfos mutants impair wound healing. These defects are accompanied by lowered or loss of expression of puc. In support of a role of puc in wound healing, hep mutant phenotypes are rescued by reducing puc function, whereas overexpression of puc inhibits wound healing. Altogether, these results demonstrate a role for the JNK pathway in imaginal disc wound healing, similar to that reported for other healing processes such as embryonic dorsal closure, thoracic closure, and adult epithelial wound healing in Drosophila. Differences with such processes are also highlighted. (c) 2005 Elsevier Inc. All rights reserved.