Impact of nitrogen fertilization and soil tillage on arbuscular mycorrhizal fungal communities in a Mediterranean agroecosystem
Impact of nitrogen fertilization and soil tillage on arbuscular mycorrhizal fungal communities in a Mediterranean agroecosystem
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DOI：
10.1016/j.soilbio.2013.09.005
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发表时间：
2013-12-01
影响因子：
9.7
通讯作者：
Giovannetti, Manuela
中科院分区：
文献类型：
--
作者：
Avio, Luciano;Castaldini, Maurizio;Giovannetti, Manuela
在地中海耕地系统中，通过结合田间土壤和微宇宙中生长的陷阱植物的土壤和根的分子、生物化学和形态学分析，研究了氮肥（N）施肥和耕作对丛枝菌根真菌（AMF）的影响。PCR DGGE带型的典范对应分析（CCA）表明，施氮和耕作对AMF群落有影响。施氮量也是影响苜蓿根系和土壤AMF群落的主要因素。CCA分析表明，不同种类AMF的产孢模式显示耕作对AMF群落的显著影响。在翻耕土壤中，藓类真菌以漏斗状菌为优势种，而在免耕土壤中，粘球囊霉和根内球囊霉为优势种。田间球囊霉素相关的土壤蛋白质含量减少耕作措施。我们的多式联运的方法，提供数据的两个主要生产因素影响土壤AMF社区，可能有助于实施有效的农业管理策略，能够支持作物和本地AMF共生体之间的有益关系。(C)2013由Elsevier Ltd.出版
The impact of nitrogen (N) fertilization and tillage on arbuscular mycorrhizal fungi (AMF) was studied in a Mediterranean arable system by combining molecular, biochemical and morphological analyses of field soil and of soil and roots from trap plants grown in microcosm. Canonical correspondence analysis (CCA) of PCR DGGE banding patterns evidenced that AMF communities in the field are affected by N-fertilization and tillage. N-fertilization was also the main factor shaping AMF communities in Medicago sativa trap plant soil and roots. The overall sporulation pattern of the different AMF species showed a predominant effect of tillage on AMF communities, as shown by CCA analysis. Funneliformis mosseae was the predominant species sporulating in tilled soils, while Glomus viscosum and Glomus intraradices prevailed in no-tilled soils. Field glomalin-related soil protein content was reduced by tillage practices. Our multimodal approach, providing data on two main production factors affecting soil AMF communities, may help implementing effective agricultural management strategies able to support the beneficial relationship between crops and native AMF symbionts. (C) 2013 Published by Elsevier Ltd.