Developmental imaging: Insights into the avian embryo.
Developmental imaging: Insights into the avian embryo.
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DOI：
10.1002/bdrc.20021
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发表时间：
2004-09-01
期刊：
影响因子：
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通讯作者：
Kulesa, Paul M
中科院分区：
文献类型：
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作者：
Kulesa, Paul M
使用不同的动物模型系统研究胚胎事件对于深入了解人类发育和出生缺陷至关重要。生物成像通过提供时空框架将复杂的细胞运动与分子数据联系起来，在这方面发挥了重要作用。然而，根据胚胎的年龄和形态发生事件的位置，可视化通常需要设计新的培养和成像技术。用于生物成像的主要模型系统之一是禽类胚胎，这是由于其易于操作、早期相对二维的形态发生以及在培养中生长时的活力。在禽类胚胎培养和细胞标记方面的重要工作，以及成像技术的进步，现在使人们有可能监测从产卵到孵化期间的许多发育事件。在这里，我们介绍了最新的鸟类发育成像，重点是细胞标记，胚胎培养和成像技术。
The study of embryonic events using different animal model systems is crucial for gaining insights into human development and birth defects. Biological imaging plays a major role in this effort by providing a spatiotemporal framework to link complex cell movements with molecular data. However, depending on the age of the embryo and the location of a morphogenetic event, visualization often requires the design of novel culture and imaging techniques. One of the primary model systems for biological imaging is the avian embryo, due to its accessibility to manipulation, relatively two-dimensional morphogenesis early on, and viability when grown in culture. Significant work in avian embryo culture and cell labeling, together with advances in imaging technology, now make it possible to monitor many developmental events within the period from egg laying to hatching. Here, we present the latest in avian developmental imaging, focusing on cell labeling, embryo culture, and imaging technologies.