Voluntary wheel running improves recovery from a moderate spinal cord injury
Voluntary wheel running improves recovery from a moderate spinal cord injury
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DOI：
10.1089/neu.2005.22.157
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发表时间：
2005-01-01
影响因子：
4.2
通讯作者：
Cotman, CW
中科院分区：
文献类型：
--
作者：
Engesser-Cesar, C;Anderson, AJ;Cotman, CW
最近，运动训练被证明可以改善脊髓损伤(SCI)患者的地面运动能力。这再次引发了人们对运动在脊髓损伤后康复中的作用的兴趣。然而，目前尚无脊髓损伤后自主运动和功能恢复的小鼠模型。在这里，我们报告说，自愿车轮跑可以改善小鼠脊髓损伤后的恢复。C57BI/10雌性小鼠在自愿车轮运行3周或标准单室条件下运行3周后，用IH冲击器造成60kdyne T9挫伤。经过7天的恢复期后，跑步的小鼠回到了他们的跑轮上。使用Basso，Beattie and Bresnahan(BBB)运动评定量表和Basso Mouse Scale(BMS)运动评定量表(最近专门为小鼠开发的一种量表)测量每周的开阔场地行为测量运动恢复。使用标准梯级轮的初步实验表明，车轮运行会损害恢复，但随后使用改进的平面轮的实验表明，通过锻炼可以提高恢复能力。脊髓损伤后14天，改良平台组的BBB和BMS评分显著高于久坐组。重复测量方差分析显示，与久坐不动的小鼠相比，改良平面跑小鼠的运动恢复明显提高(p<；0.05)。使用梯形横梁任务进行的运动评估也显示改进后的平板跑道有显著的改善(p<；0.05)。最后，纤维连接蛋白染色显示，两组之间的病变大小没有显著差异。这些数据代表了第一个表明自愿锻炼改善脊髓损伤后恢复的小鼠模型。
Recently, locomotor training has been shown to improve overground locomotion in patients with spinal cord injury (SCI). This has triggered renewed interest in the role of exercise in rehabilitation after SCI. However, there are no mouse models for voluntary exercise and recovery of function following SCI. Here, we report voluntary wheel running improves recovery from a SCI in mice. C57BI/10 female mice received a 60-kdyne T9 contusion injury with an IH impactor after 3 weeks of voluntary wheel running or 3 weeks of standard single housing conditions. Following a 7-day recovery period, running mice were returned to their running wheels. Weekly open-field behavior measured locomotor recovery using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale and the Basso Mouse Scale (BMS) locomotor rating scale, a scale recently developed specifically for mice. Initial experiments using standard rung wheels show that wheel running impaired recovery, but subsequent experiments using a modified flat-surface wheel show improved recovery with exercise. By 14 days post SCI, the modified flat-surface running group had significantly higher BBB and BMS scores than the sedentary group. A repeated measures ANOVA shows locomotor recovery of modified flat-surface running mice was significantly improved compared to sedentary animals (p < 0.05). Locomotor assessment using a ladder beam task also shows a significant improvement in the modified flat-surface runners (p < 0.05). Finally, fibronectin staining shows no significant difference in lesion size between the two groups. These data represent the first mouse model showing voluntary exercise improves recovery after SCI.