Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus
Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus
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DOI：
10.1002/adma.200700371
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发表时间：
2007-10-05
影响因子：
29.4
通讯作者：
Anderson, Daniel G.
中科院分区：
文献类型：
--
作者：
Green, Jordan J.;Zugates, Gregory T.;Anderson, Daniel G.
末端修饰的聚（β-氨基酯）是一种易于合成的可降解聚合物，能够将DNA递送到原代人类细胞，其水平与腺病毒相当，比常用的非病毒载体聚乙烯亚胺高两个数量级。发现微小的结构变化会影响基因递送的多个步骤，包括DNA结合亲和力、纳米颗粒尺寸、细胞内DNA吸收和最终蛋白质表达。在体内，这些聚合物修饰增强了DNA向卵巢肿瘤的递送。
End-modified poly(beta-amino ester)s, easy-to-synthesize degradable polymers, are able to deliver DNA to primary human cells at levels comparable to adenovirus and two orders of magnitude better than the commonly used non-viral vector, polyethylenimine. Small structural changes are found to affect multiple steps of gene delivery including the DNA binding affinity, nanoparticle size, intracellular DNA uptake, and final protein expression. In vivo, these polymer modifications enhance DNA delivery to ovarian tumors.