hsp108,,,,,, a novel heat shock inducible protein of chicken.
hsp108,,,,,, a novel heat shock inducible protein of chicken.
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DOI：
10.1021/bi00368a062
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发表时间：
1986-10
期刊：
影响因子：
2.9
通讯作者：
D. Sargan;M. Tsai;B. O’Malley
中科院分区：
文献类型：
--
作者：
D. Sargan;M. Tsai;B. O’Malley
已经描述了编码与孕酮受体B亚单位相互作用但不与孕酮结合的蛋白质的cDNA克隆[Kulomaa，M.S.，Weigel，N.L.，Kleinsek，D.A.，Beattie，W.G.，Conneely，O.M.，March，C.，Zarucki-Schulz，T.，Schrader，W.T.，&O‘Malley，B.W.(1986)生物化学(本期的前一篇论文)]。获得了这些克隆的全长序列，并发现其编码的蛋白质在结构上与孕酮受体无关，但与先前描述的酵母热休克蛋白HSP90和果蝇HSP83a具有显著的同源性。本文证明该蛋白确实是一种热休克蛋白。虽然该蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶上的表观分子量为108,000，但多肽骨架的分子量为92,000。热休克可以诱导基因转录物的稳定水平和蛋白质的水平，但该基因在许多组织中是结构性表达的。还报道了这些制剂中一种以前未描述的热休克蛋白，其分子量为78,000。
cDNA clones encoding a protein that copurifies with the progesterone receptor B subunit but does not bind progesterone have been described [Kulomaa, M. S., Weigel, N. L., Kleinsek, D. A., Beattie, W. G., Conneely, O. M., March, C., Zarucki-Schulz, T., Schrader, W. T., & O'Malley, B. W. (1986) Biochemistry (preceding paper in this issue)]. A full-length sequence for these clones was derived and was found to encode a protein that is structurally unrelated to the progesterone receptor but that contains significant homologies to the previously described heat shock proteins hsp90 of yeast and hsp83a of Drosophila melanogaster. In this paper it is shown that this protein is indeed a heat shock protein. Though the apparent molecular weight of the protein is 108,000 on sodium dodecyl sulfate-polyacrylamide gels, the molecular weight of the polypeptide backbone is 92,000. The steady-state level of gene transcripts as well as the level of protein is inducible by heat shock, but the gene is constitutively expressed in a number of tissues. A previously undescribed heat shock protein of molecular weight 78,000 in these preparations is also reported.