Inhibition studies of alkaline phosphatase in hard tissue-forming cells.
Inhibition studies of alkaline phosphatase in hard tissue-forming cells.
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硬组织形成细胞中碱性磷酸酶的抑制研究。
DOI：
10.1177/23.5.165233
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
B. Magnusson
中科院分区：
文献类型：
--
作者：
A. Linde;B. Magnusson
研究了碱性磷酸酶抑制剂左旋咪唑和R8231对牙本质活性成牙本质细胞中对硝基苯基磷酸酶、无机焦磷酸酶和三磷酸腺苷酶(ATPase)活性的影响。对硝基苯基磷酸酶和无机焦磷酸酶活性受到抑制，而ATP裂解酶活性仍保持在40%的水平。这一发现与早期的研究一起表明，在硬组织形成细胞中，至少有两种不同的磷酸酶在碱性pH下具有活性；非特异性碱性磷酸酶和一种特异性ATPase。该酶活性不受哇巴因和Ru红的影响，可被Ca~(2+)激活。
The effects of the alkaline phosphatase inhibitors levamisole and R 8231 on p-nitro-phenylphosphatase, inorganic pyrophosphatase and adenosine triphosphatase (ATPase) activities in dentingenically active odontoblasts were studied. The p-nitrophenylphosphatase and inorganic pyrophosphatase activities were inhibited, while 40% of the ATP-splitting enzyme activity remained under the assay condition used. This finding, togeather with earlier studies, indicates that at least two different phosphatase are active at alkaline pH in hard tissue-forming cells; on nonspecific alkaline phosphatase and one specific ATPase. The ATPase activity is uninfluenced by ouabain and ruthenium red and is activated by Ca-2+ ions.