Preparation of autologous antiserum against SSV nonproducer cells and its partial characterization.
Preparation of autologous antiserum against SSV nonproducer cells and its partial characterization.
复制标题
针对 SSV 非生产细胞的自体抗血清的制备及其部分表征。
DOI：
10.1016/0042-6822(81)90308-1
复制
发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Broughton,EM
中科院分区：
文献类型：
--
作者：
Thiel,HJ;Iglehart,JD;Matthews,TJ;Broughton,EM
为了制备针对SSV非产生细胞（SSV- np细胞）的自体抗血清，将活组织标本中的山羊成纤维细胞感染SSV (SSAV)，并分离转化的非产生细胞克隆。其中一个克隆在山羊自体血清中繁殖，并接种到同一只山羊身上。通过表面特异性结合试验对所得抗血清进行了表征，结果显示抗血清与NRK-SSV非产生细胞具有特异性反应性，而NRK-SSV非产生细胞不能被SSAV病毒树脂吸附。这些数据表明血清可以识别细胞表面分子，这可能是SSV转化的特异性分子。
In order to prepare an autologous antiserum against SSV nonproducer cells (SSV-NP cells), goat fibroblasts from a biopsy specimen were infected with SSV (SSAV) and transformed nonproducer clones were isolated. One of these clones was propagated in autologous goat serum and inoculated into the same goat. Characterization of the resulting antiserum by a surface specific binding assay showed specific reactivity with NRK-SSV nonproducer cells, which cannot be adsorbed by an SSAV virus resin. The data suggest that the serum recognizes cell surface molecules, which might be specific for transformation by SSV.