Epidemiological and molecular evidence supports the zoonotic transmission of Glardia amongy humans and dogs living in the same community
Epidemiological and molecular evidence supports the zoonotic transmission of Glardia amongy humans and dogs living in the same community
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DOI：
10.1017/s0031182003004505
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发表时间：
2004-03-01
期刊：
影响因子：
2.4
通讯作者：
Thompson, RCA
中科院分区：
文献类型：
--
作者：
Traub, RJ;Monis, PT;Thompson, RCA
从生活在印度东北部偏远茶园社区同一地点的人类和木屐中回收的十二指肠贾第鞭毛虫分离株在 3 个不同的位点进行了表征； SSU-rDNA、延伸因子 1-α (ef1-α) 和磷酸丙糖异构酶 (tpi) 基因。 SSU-rDNA 和 ef1-alpha 基因的系统发育分析提供了各种组合内分离株的较差遗传分辨率，强调了在推断贾第鞭毛虫基因型时使用多个基因座的重要性。对 tpi 基因的分析提供了更好的遗传分辨率，并将犬贾第鞭毛虫分离株置于人类分离株的遗传分组内（组合 A 和 B）。流行病学数据支持了人畜共患传播的进一步证据，显示人类贾第鞭毛虫患病率与同一家庭中贾第鞭毛虫阳性狗的存在之间存在高度显着相关性（比值比 3.01, 95%）CI, 1.11, 8.39, P = 0.0000）。
Giardia duodenalis isolates recovered from humans and clogs living in the same locality in a remote tea-growing community of northeast India were characterized at 3 different loci; the SSU-rDNA, elongation factor 1-alpha (ef1-alpha) and triose phosphate isomerase (tpi) gene. Phylogenetic analysis of the SSU-rDNA and ef1-alpha genes provided poor genetic resolution of the isolates within various assemblages, stressing the importance of using multiple loci when inferring genotypes to Giardia. Analysis of the tpi gene provided better genetic resolution and placed canine Giardia isolates within the genetic groupings of human isolates (Assemblages A and B). Further evidence for zoonotic transmission was supported by epidemiological data showing a highly significant association between the prevalence of Giardia in humans and presence of it Giardia-positive dog in the same household (odds ratio 3.01, 95%) CI, 1.11, 8.39, P = 0.0000).