OPAQUE3, encoding a transmembrane bZIP transcription factor, regulates endosperm storage protein and starch biosynthesis in rice.
OPAQUE3, encoding a transmembrane bZIP transcription factor, regulates endosperm storage protein and starch biosynthesis in rice.
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DOI：
10.1016/j.xplc.2022.100463
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发表时间：
2022-11-14
影响因子：
10.5
通讯作者：
Hu, Peisong
中科院分区：
文献类型：
--
作者：
Cao, Ruijie;Zhao, Shaolu;Jiao, Guiai;Duan, Yingqing;Ma, Liuyang;Dong, Nannan;Lu, Feifei;Zhu, Mingdong;Shao, Gaoneng;Hu, Shikai;Sheng, Zhonghua;Zhang, Jian;Tang, Shaoqing;Wei, Xiangjin;Hu, Peisong
淀粉和贮藏蛋白是稻米的主要成分。谷物。尽管它们很重要，但贮藏蛋白质和淀粉生物合成的分子调控机制在很大程度上仍然难以捉摸。在这里，我们鉴定了一个水稻不透明胚乳突变体，opaque3(03)，它过量积累57 kDa的谷蛋白，蛋白质和淀粉含量显著低于野生型。突变体的胚乳细胞中还存在蛋白质小体结构异常和复合淀粉粒。OPAQUE3(O3)编码一种跨膜碱性亮氨酸拉链(BZIP)转录因子(OsbZIP60)，定位于内质网(ER)和细胞核，但在ER应激下主要定位于细胞核。我们发现，臭氧可以激活几个淀粉合成相关基因(GBSSI、AGPL2、SBEI和ISA2)和贮藏蛋白合成相关基因(OsGluA2、ProL14和Glb1)的表达。臭氧还通过直接与启动子结合并激活水稻胚乳细胞内质网中两个主要的伴侣蛋白OsBIP1和PDIL1-1的表达，在内质网中发挥重要的蛋白质加工和输出作用。高温条件加剧了内质网胁迫，导致了臭氧突变体中更多的异常籽粒发育。我们还揭示了OsbZIP50可以帮助臭氧响应内质网胁迫，特别是在高温条件下。因此，我们证明了臭氧在水稻籽粒发育中起着核心作用，它通过同时参与调节胚乳细胞中贮藏蛋白质和淀粉的生物合成以及维持内质网的动态平衡。OPAQUE3(O3)编码一种跨膜bZIP转录因子，在内质网(ER)胁迫下重新定位到细胞核。在水稻籽粒发育过程中，臭氧通过参与调节胚乳细胞内贮藏蛋白质和淀粉的生物合成以及维持内质网的稳态，在水稻籽粒发育过程中起着核心作用。
Starch and storage proteins are the main components of rice (Oryza sativa L.) grains. Despite their importance, the molecular regulatory mechanisms of storage protein and starch biosynthesis remain largely elusive. Here, we identified a rice opaque endosperm mutant, opaque3 (o3), that overaccumulates 57-kDa proglutelins and has significantly lower protein and starch contents than the wild type. The o3 mutant also has abnormal protein body structures and compound starch grains in its endosperm cells. OPAQUE3 (O3) encodes a transmembrane basic leucine zipper (bZIP) transcription factor (OsbZIP60) and is localized in the endoplasmic reticulum (ER) and the nucleus, but it is localized mostly in the nucleus under ER stress. We demonstrated that O3 could activate the expression of several starch synthesis-related genes (GBSSI, AGPL2, SBEI, and ISA2) and storage protein synthesis-related genes (OsGluA2, Prol14, and Glb1). O3 also plays an important role in protein processing and export in the ER by directly binding to the promoters and activating the expression of OsBIP1 and PDIL1-1, two major chaperones that assist with folding of immature secretory proteins in the ER of rice endosperm cells. High-temperature conditions aggravate ER stress and result in more abnormal grain development in o3 mutants. We also revealed that OsbZIP50 can assist O3 in response to ER stress, especially under high-temperature conditions. We thus demonstrate that O3 plays a central role in rice grain development by participating simultaneously in the regulation of storage protein and starch biosynthesis and the maintenance of ER homeostasis in endosperm cells. OPAQUE3 (O3) encodes a transmembrane bZIP transcription factor that relocates to the nucleus under endoplasmic reticulum (ER) stress. O3 plays a central role in rice grain development by participating in the regulation of storage protein and starch biosynthesis and the maintenance of ER homeostasis in endosperm cells.
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DOI：
10.1073/pnas.0408941102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Iwata, Y;Koizumi, N
通讯作者：
Koizumi, N
谷蛋白前体累积3编码对水稻胚乳液泡蛋白分选至关重要的后高尔基体囊泡运输调节因子
影响因子：
11.6
作者：
Ren, Yulong;Wang, Yihua;Wan, Jianmin
通讯作者：
Wan, Jianmin
影响因子：
56.9
作者：
LI, XX;WU, YJ;OKITA, TW
通讯作者：
OKITA, TW
影响因子：
7.4
作者：
Pan, Tian;Wang, Yihua;Wan, Jianmin
通讯作者：
Wan, Jianmin
影响因子：
13.8
作者：
Bello, Babatunde Kazeem;Hou, Yuxuan;Zhang, Jian
通讯作者：
Zhang, Jian