RFLP analysis of nuclear DNA for study of phylogeny and domestication of tetraploid wheat
RFLP analysis of nuclear DNA for study of phylogeny and domestication of tetraploid wheat
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核 DNA 的 RFLP 分析用于四倍体小麦的系统发育和驯化研究
DOI：
10.1266/ggs.72.153
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发表时间：
1997
影响因子：
1.1
通讯作者：
C. Nakamura
中科院分区：
文献类型：
--
作者：
N. Mori;T. Moriguchi;C. Nakamura
为了研究四倍体小麦的系统发育和驯化，采用RFLP分析方法研究了四倍体小麦栽培种和野生种的核DNA变异。采用29种限制性内切酶组合和20个探针，对11种栽培四倍体小麦（Emmer小麦和Timopheevi小麦）、16种野生Emmer小麦（Triticum dicoccoides Korn.）、14种野生Timopheevi小麦（T. araraticum Jakubz.）和1种普通小麦（T. aestivum）进行了分析。在此基础上，估计了各对材料之间的遗传距离(d)。Emmer组平均d为0.0189，Timopheevi组平均d为0.0024，组间平均d为0.0698。利用UPGMA、NJ （neighbor joining）和最大简约法进行聚类分析，发现Emmer小麦和Timopheevi小麦分化明显。在栽培的二粒小麦中。其核苷酸多样性最大（π = 0.0180），与野生祖先种T. dicoccoides（0.0186）接近。所有栽培种属，除白绒T. dicoccum和paleocolchicum Men外。，在系统发育树中被归类为一个不同的簇。除一个品种外，其余品种均归为另一个品种。小麦是一种非自由脱粒的小麦品种，其巨大的遗传多样性支持了小麦是最早驯化的四倍体小麦的考古证据。
To study the phylogeny and domestication of tetraploid wheat species, variations in nuclear DNA of the cultivated and wild species were investigated by RFLP analysis. Twenty-two accessions representing 11 species of cultivated tetraploid wheat (Emmer wheat and Timopheevi wheat), 16 accessions of wild Emmer wheat (Triticum dicoccoides Korn.), 14 accessions of wild Timopheevi wheat (T. araraticum Jakubz.), and an accession of common wheat (T. aestivum) were analyzed, using 29 combinations of two restriction enzymes and 20 probes. Based on this result, the genetic distances (d) between all pairs of accessions were estimated. An average d was 0.0189 in the Emmer group and 0.0024 in the Timopheevi group, while that between the groups was 0.0698. Cluster analysis using UPGMA, NJ (neighbor joining) and maximum parsimony method showed clear differentiation of Emmer wheat and Timopheevi wheat. Among the cultivated Emmer wheat T. dicoccum Schubl. showed the largest nucleotide diversity (π = 0.0180) which was close to that (0.0186) in the wild ancestral species, T. dicoccoides. All the cultivated species, except for T. dicoccum and T. paleocolchicum Men., were grouped into a distinct cluster in the phylogenetic trees. All but one accessions of T. dicoccoides were grouped in another. The large genetic diversity in T. dicoccum, the non-free threshing species, supports the archeological evidence that T. dicoccum was the earliest domesticated tetraploid wheat.