A comparison of the Coulter STKS, Coulter S+IV, and manual analysis of white blood cell differential counts in a human immunodeficiency virus-infected population.
A comparison of the Coulter STKS, Coulter S+IV, and manual analysis of white blood cell differential counts in a human immunodeficiency virus-infected population.
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Coulter STKS、Coulter S IV 和人类免疫缺陷病毒感染人群白细胞分类计数手动分析的比较。
DOI：
10.1093/ajcp/100.6.611
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发表时间：
1993
影响因子：
3.5
通讯作者：
Steigbigel,RT
中科院分区：
文献类型：
--
作者：
Cohen,AJ;Peerschke,EI;Steigbigel,RT
在人类免疫缺陷病毒（HIV）感染人群中，比较了通过手动分析和使用Coulter STKS和Coulter S+IV细胞计数器（Coulter Electronics，希亚利亚，FL）自动分析获得的白色血细胞分类计数。线性相关分析表明，在淋巴细胞和粒细胞计数的测定中，STKS的准确性低于S+IV。通过差异分析和确定超出95%置信限的测量值数量证实了这一发现。对不知道是否感染HIV的患者也进行了类似的分析，在该组中STKS和S+IV之间没有发现差异。在HIV感染人群中，在STKS上进行的手动和自动粒细胞计数之间的差异随着红细胞平均体积的增加而增加，但在S+IV上没有。对HIV感染患者进行研究的医生和研究者应注意在使用STKS分类时可能出现假性低粒细胞数。
The white blood cell differential counts obtained by manual analysis and those obtained by automated analysis using Coulter STKS and Coulter S+IV cell counters (Coulter Electronics, Hialeah, FL) in a human immunodeficiency virus (HIV)-infected population were compared. Linear correlation analyses showed the STKS to be less accurate than the S+IV in determinations of the lymphocyte and granulocyte count. This finding was confirmed by difference analyses and by determining the number of measurements that fell outside of 95% confidence limits. Similar analyses were also done on patients not known to be HIV infected, and there was no disparity found between the STKS and S+IV in that group. In the HIV-infected population, the discrepancy between the manual and automated granulocyte counts performed on the STKS, but not on the S+IV, increased with increasing red blood cell mean corpuscular volume. Physicians caring for, and investigators performing studies on, HIV-infected patients should be aware of the potential for falsely low granulocyte numbers when using the differential from the STKS.