SEQUENTIAL EXPRESSION AND DIFFERENTIAL LOCALIZATION OF I-FIMBRIN, L-FIMBRIN, AND T-FIMBRIN DURING DIFFERENTIATION OF THE MOUSE INTESTINE AND YOLK-SAC
SEQUENTIAL EXPRESSION AND DIFFERENTIAL LOCALIZATION OF I-FIMBRIN, L-FIMBRIN, AND T-FIMBRIN DURING DIFFERENTIATION OF THE MOUSE INTESTINE AND YOLK-SAC
复制标题
DOI：
10.1002/aja.1002030203
复制
发表时间：
1995-06-01
影响因子：
2.5
通讯作者：
MATSUDAIRA, P
中科院分区：
文献类型：
--
作者：
CHAFEL, MM;SHEN, WY;MATSUDAIRA, P
在肠上皮分化过程中，绒毛蛋白、纤维蛋白和肌球蛋白I这三种细胞骨架蛋白依次表达并定位于顶膜。最近，我们发现在成年小鼠和人类中，三种纤维蛋白异构体以细胞特异性的方式表达。i -纤维蛋白在肠和肾上皮细胞中表达，l -纤维蛋白在白细胞和多种肿瘤中表达，而t -纤维蛋白在多种细胞和组织中表达。由于非肠型纤维蛋白可以在发育早期表达，因此我们研究了在肠发育10.5 ~ 16.5天期间纤维蛋白异构体的表达。免疫荧光显微镜观察发现，在肠上皮细胞分化的早期，直到第14.5天，就可以检测到t -纤蛋白，并且主要位于肠上皮细胞的根尖表面。在此期间也检测到l -纤蛋白，但它定位于上皮的基底表面而不是顶端表面。到第16.5天，上皮内未检测到L或t -纤维蛋白。第14.5天首次检测到i -纤蛋白，第16.5天出现刷状边缘样根尖定位模式。与肠细胞不同，内脏内胚层在整个检查期间表达I、L和t -纤维蛋白，随着时间的推移，I-纤维蛋白的水平增加。l -纤连蛋白在发育早期比后期更为明显。总之，这些结果表明，三种纤蛋白亚型在上皮细胞分化过程中起着不同的作用。T-和i -纤维蛋白的表达可能对微绒毛的形成和延伸至关重要，而l -纤维蛋白可能在控制细胞粘附方面发挥作用。(C) 1995 Wiley-Liss, Inc。
During the differentiation of the intestine epithelium, three cytoskeletal proteins, villin, fimbrin, and myosin I, are sequentially expressed and localized to the apical membrane. Recently, we found that in the adult mouse and human, three fimbrin isoforms are expressed in a cell specific manner. I-fimbrin is expressed by intestine and kidney epithelial cells, L-fimbrin is expressed by leukocytes and many tumors, while T-fimbrin is expressed by various cells and tissues, Because non-intestinal isoforms of fimbrin could be expressed early in development, the expression of fimbrin isoforms during days 10.5 to 16.5 of intestine development was investigated. By immunofluorescence microscopy, T-fimbrin was detected in the early stages of intestinal epithelial cell differentiation until day 14.5 and was localized predominantly at the apical surface. L-fimbrin was also detected during this period but it was localized to the basal surface of the epithelium instead of the apical surface. By day 16.5 no L or T-fimbrin was detected in the epithelium. I-fimbrin was first detected at day 14.5 and a brush border-like apical localization pattern was seen by day 16.5. Unlike the intestinal cells, the visceral endoderm expressed I, L, and T-fimbrin throughout the period examined, with the level of I-fimbrin increasing as time progresses. L-fimbrin was more evident at the earlier stage than at the later stage of the development. Collectively, these results suggest that three fimbrin isoforms play different roles during epithelial cell differentiation. T- and I-fimbrin expression could be critical for the formation and extension of the microvilli whereas L-fimbrin may play a role in controlling cell adhesion. (C) 1995 Wiley-Liss, Inc.