Fluorescence biosensing in selectively photo-activated microbubble resonators
Fluorescence biosensing in selectively photo-activated microbubble resonators
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DOI：
10.1016/j.snb.2016.09.146
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发表时间：
2017-04-01
影响因子：
8.4
通讯作者：
Giannetti, Ambra
中科院分区：
文献类型：
--
作者：
Berneschi, Simone;Baldini, Francesco;Giannetti, Ambra
本文报道了一种利用光化学活化在光学微泡共振器（OMBRs）内表面上实现抗体空间选择性固定的方法。该方法基于合适的UV掩模工艺。作为该技术概念的证明，在光活化OMBR中实施免疫测定并进行荧光研究。此外，这一过程的可能应用对基于OMBR的生化多重检测系统的发展。最后，在免疫测定事件之后测量的高Q因子值（>10（5））使得这些光活化的OMBR适合用于未来的无标记生物传感器实施的装置。(C)© 2016 Elsevier B.V.版权所有。
A procedure for a spatially selective immobilization of antibodies, via photochemical activation, on the inner surface of optical microbubble resonators (OMBRs) is reported. The method is based on a suitable UV mask process. As proof of concept of this technique, immunoassay in photo-activated OMBR was implemented and fluorescence investigation was conducted. Moreover, the possible application of this procedure towards the development of a biochemical multiplexed detection system based on OMBR is presented. Finally, the high Q factor values measured after the immunoassay event (>10(5)) make these photo-activated OMBRs suitable devices for future label-free biosensor implementation. (C) 2016 Elsevier B.V. All rights reserved.