A Mitochondria-Targetable Fluorescent Probe for Dual-Channel NO Imaging Assisted by Intracellular Cysteine and Glutathione
A Mitochondria-Targetable Fluorescent Probe for Dual-Channel NO Imaging Assisted by Intracellular Cysteine and Glutathione
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细胞内半胱氨酸和谷胱甘肽辅助的用于双通道 NO 成像的线粒体靶向荧光探针
DOI：
10.1021/ja504156a
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发表时间：
2014-09-10
影响因子：
15
通讯作者：
Guo, Wei
中科院分区：
文献类型：
--
作者：
Sun, Yuan-Qiang;Liu, Jing;Guo, Wei
将焦宁染料与邻苯二胺（OPD）的氨基直接偶联，合成了一种特异性的NO（1）荧光探针。该探针可以选择性地检测NO超过脱氢抗坏血酸（DHA），抗坏血酸（AA），和甲基乙二醛（MGO），以及活性氧/氮物种（ROS/RNS）与显着的关-开响应，由于生产的红色发射三唑2。在半胱氨酸/谷胱甘肽（Cys/GSH）的存在下，2可以进一步转化为发绿光的氨基吡喃4和发红光的硫吡喃5，分别。在胞内Cys和GSH的辅助下，该探针显示了其在双通道模式下监测线粒体NO的潜力。
A mitochondria-specific fluorescent probe for NO (1) was synthesized by the direct conjugation of a pyronin dye with one of the amino groups of o-phenylenediamino (OPD). The probe could selectively detect NO over dehydroascorbic acid (DHA), ascorbic acid (AA), and methylglyoxal (MGO) as well as the reactive oxygen/nitrogen species (ROS/RNS) with the significant off-on response due to the production of a red-emission triazole 2. In the presence of cysteine/glutathione (Cys/GSH), 2 could be further transformed into a green-emission aminopyronin 4 and a red-emission thiopyronin 5, respectively. Assisted by intracellular Cys and GSH, the probe demonstrated its potential to monitor mitochondrial NO in a dual-channel mode.