Persistence of Streptococcus mutans in stationary-phase batch cultures and biofilms.
Persistence of Streptococcus mutans in stationary-phase batch cultures and biofilms.
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变形链球菌在固定相分批培养物和生物膜中的持久性。
DOI：
10.1128/aem.70.10.6181-6187.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Buttaro,BettinaA
中科院分区：
文献类型：
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作者：
RenyeJr,JohnA;Piggot,PatrickJ;Daneo-Moore,Lolita;Buttaro,BettinaA
变形链球菌是口腔菌斑生物膜的一员，被认为是龋病的主要病原体。我们已经描述了S的生存。突变株UA 159在分批培养和生物膜中的表达。在含有过量葡萄糖或蔗糖的化学成分确定的培养基FMC中分批培养生长的细菌在进入稳定期时导致pH降低至4.0，并且它们存活约3天。当培养基中含有限制浓度的葡萄糖或蔗糖时，细菌达到稳定期时，葡萄糖或蔗糖被耗尽，存活期延长至11天。糖限制培养物在整个稳定期保持7.0的pH。在进入稳定期时加入外源性乳酸可使其存活时间缩短至3天。糖饥饿没有导致生物膜中的可比存活率。尽管pH保持在7.0，但在施加葡萄糖或蔗糖饥饿后4天，细菌不再能从生物膜中培养出来;BacLight染色结果与基于可培养性的存活结果不一致。在分批培养和生物膜中，通过向培养基中添加0.5%粘蛋白可以延长存活。批次存活增加到平均26（±8）天，并且在蔗糖饥饿12天的生物膜中仍然存在平均2.7 × 105 CFU/室。
Streptococcus mutansis a member of oral plaque biofilms and is considered the major etiological agent of dental caries. We have characterized the survival ofS. mutansstrain UA159 in both batch cultures and biofilms. Bacteria grown in batch cultures in a chemically defined medium, FMC, containing an excess of glucose or sucrose caused the pH to decrease to 4.0 at the entry into stationary phase, and they survived for about 3 days. Survival was extended up to 11 days when the medium contained a limiting concentration of glucose or sucrose that was depleted by the time the bacteria reached stationary phase. Sugar-limited cultures maintained a pH of 7.0 throughout stationary phase. Their survival was shortened to 3 days by the addition of exogenous lactic acid at the entry into stationary phase. Sugar starvation did not lead to comparable survival in biofilms. Although the pH remained at 7.0, bacteria could no longer be cultured from biofilms 4 days after the imposition of glucose or sucrose starvation;BacLight staining results did not agree with survival results based on culturability. In both batch cultures and biofilms, survival could be extended by the addition of 0.5% mucin to the medium. Batch survival increased to an average of 26 (±8) days, and an average of 2.7 × 105CFU per chamber were still present in biofilms that were starved of sucrose for 12 days.