NATURAL METHICILLIN RESISTANCE IN COMPARISON WITH THAT SELECTED BY INVITRO DRUG EXPOSURE IN STAPHYLOCOCCUS-AUREUS
NATURAL METHICILLIN RESISTANCE IN COMPARISON WITH THAT SELECTED BY INVITRO DRUG EXPOSURE IN STAPHYLOCOCCUS-AUREUS
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DOI：
10.1093/jac/23.2.179
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发表时间：
1989-02-01
影响因子：
5.2
通讯作者：
KAYSER, FH
中科院分区：
文献类型：
--
作者：
BERGERBACHI, B;STRASSLE, A;KAYSER, FH
将在递增浓度的甲氧西林中连续传代期间选择的甲氧西林耐药葡萄球菌与携带在金黄色葡萄球菌临床分离株中发现的甲氧西林耐药决定簇（mec）的同基因甲氧西林耐药菌株进行比较。选择甲氧西林耐药性之后，青霉素结合蛋白（PBPs）2和4的结合特性发生变化。在mec确定的低亲和力PBP2“和体外选择的突变体的PBPs之间没有免疫学或遗传学关系。Tn551插入（Ω 2003，其使mec确定的甲氧西林抗性失活，但与mec无关）也部分降低了体外选择的甲氧西林抗性。这表明，这种甲氧西林耐药性控制因素至少部分有助于体外选择的甲氧西林耐药性。
Methicillin resistant staphylococci selected during serial passage in increasing concentrations of methicillin were compared with an isogenic methicillin resistant strain carrying the methicillin resistance determinant (mec) found in clinical isolates of Staphylococcus aureus. Selection for methicillin resistance was followed by changes in the binding characteristic of penicillin binding proteins (PBPs) 2 and 4. There was no immunological nor genetic relationship between the mec-determined low affinity PBP2'' and the PBPs of the in-vitro selected mutants. The Tn551 insertion (.OMEGA.2003, which inactivates mec determined methicillin resistance, but which is not linked to mec, also partially reduced in-vitro selected methicillin resistance. This suggested that this methicillin resistance controlling factor contributes at least partially to methicillin resistance selected in vitro.