Differential non-target-derived repulsive signals play a critical role in shaping initial axonal growth of dorsal root ganglion neurons
Differential non-target-derived repulsive signals play a critical role in shaping initial axonal growth of dorsal root ganglion neurons
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DOI：
10.1016/s0012-1606(02)00087-8
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发表时间：
2003-02-15
影响因子：
2.7
通讯作者：
Shiga, T
中科院分区：
文献类型：
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作者：
Masuda, T;Tsuji, H;Shiga, T
背根神经节 (DRG) 轴突的初始轨迹是由周围组织的化学脉冲信号决定的。尽管我们之前已经证明 DRG 轴突上的 axonin-1/SC2 表达需要介导脊索衍生的化学排斥信号 (T. Masuda et al., 2000, Dev. Biol. 224, 112-121)，但参与非靶标衍生的排斥信号的其他分子在很大程度上是未知的。使用由鸡胚或经功能阻断抗体和磷脂酰肌醇特异性磷脂酶 C 处理的突变小鼠组织组成的共培养测定，我们在此报告，化学排斥信号蛋白 3A (Sema3A) 及其受体神经毡蛋白-1 是介导皮肌刀和脊索衍生的 DRG 轴突化学脉冲信号所必需的，但不是腹侧脊髓衍生的化学脉冲信号。皮肌刀衍生的化学脉冲完全依赖于 Sema3A/neuropilin-1，而其他分子也参与脊索衍生的化学脉冲。来自腹侧脊髓的化学排斥不依赖于 Sema3A/neuropilin-1，但需要 axonin-1/SC2 来排斥 DRG 轴突。因此，差分化学脉冲信号有助于塑造 DRG 轴突的初始轨迹，对于神经系统的正确连接至关重要。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Initial trajectories of dorsal root ganglion (DRG) axons are shaped by chemorepulsive signals from surrounding tissues. Although we have previously shown that axonin-1/SC2 expression on DRG axons is required to mediate a notochord-derived chemorepulsive signal (T. Masuda et al., 2000, Dev. Biol. 224, 112-121), other molecules involved in the non-target-derived repulsive signals are largely unknown. Using coculture assays composed of tissues derived from the chick embryo or mutant mice treated with function-blocking antibodies and phosphatidylinositol-specific phospholipase C, we report here that the chemorepellent semaphorin 3A (Sema3A) and its receptor neuropilin-1 are required for mediating the dermamyotome- and notochord-derived, but not the ventral spinal cord-derived, chemorepulsive signal for DRG axons. The dermamyotome-derived chemorepulsion is exclusively dependent on Sema3A/neuropilin-1, whereas other molecules are also involved in the notochord-derived chemorepulsion. Chemorepulsion from the ventral spinal cord does not depend on Sema3A/neuropilin-1 but requires axonin-1/SC2 to repel DRG axons. Thus, differential chemorepulsive signals help shape the initial trajectories of DRG axons and are critical for the proper wiring of the nervous system. (C) 2003 Elsevier Science (USA). All rights reserved.