Coherent anti-Stokes Raman scattering for label-free biomedical imaging
Coherent anti-Stokes Raman scattering for label-free biomedical imaging
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DOI：
10.1088/2040-8978/15/9/094006
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发表时间：
2013-09-01
影响因子：
2.1
通讯作者：
Mahajan, Sumeet
中科院分区：
文献类型：
--
作者：
Patel, Imran I.;Steuwe, Christian;Mahajan, Sumeet
相干反斯托克斯拉曼散射（汽车）已经确立了自己作为一种成像技术，能够通过内源性分子的振动相干性提供生物样品的视频速率成像。目前的技术主要涉及应用昂贵的、侵入性的和潜在的非特异性染料或标记来对生物分子进行成像。然而，汽车显微镜可以提供一种高分辨率和非侵入性的替代方法，用于成像感兴趣的生物分子，而不需要外源标记。在这里，我们提供了汽车的概述，包括技术和常见的仪器，以及它在生物医学成像中的应用。我们讨论了汽车已应用于的主要生物医学领域，例如评估肝脏疾病，动脉粥样硬化进展，肿瘤分类和跟踪药物递送，同时还评估了临床翻译的未来挑战。
Coherent anti-Stokes Raman scattering (CARS) has established itself as an imaging technique capable of providing video-rate imaging of biological specimens through vibrational coherence of endogenous molecules. Current techniques predominantly involve the application of costly, invasive and potentially non-specific dyes or labels for imaging biomolecules. CARS microscopy can however provide a high-resolution and non-invasive alternative for imaging biomolecules of interest without the need for exogenous labels. Here we provide an overview of CARS including the technique and common instrumentation as well as its applications in biomedical imaging. We discuss the major biomedical areas where CARS has been applied such as in evaluating liver disease, progression of atherosclerosis, tumour classification and tracking drug delivery, whilst also assessing the future challenges for clinical translation.