Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers
Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers
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DOI：
10.1021/nn800034w
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发表时间：
2008-04-01
期刊：
影响因子：
17.1
通讯作者：
Orr, Bradford G.
中科院分区：
文献类型：
--
作者：
Landmark, Kevin J.;DiMaggio, Stassi;Orr, Bradford G.
合成了有机包覆的超顺磁性氧化铁纳米粒子（OC-SPION），并通过透射电子显微镜和 X 射线光电子能谱进行了表征。使用用 6-TAMRA 荧光染料和叶酸分子修饰的聚（酰胺基胺）树枝状聚合物将 OC-SPION 从有机介质转移到水中。使用超导量子干涉装置测定所得树枝状聚合物涂覆的 SPION (DC-SPION) 的饱和磁化强度为 60 emu/g Fe，而块状磁铁矿的饱和磁化强度为 90 emu/g Fe。使用两种不同且互补的成像方式：紫外可见光和 X 射线荧光，证明并量化了 DC-SPION 在体外对 KB 癌细胞的选择性靶向。共聚焦显微镜证实了内化。使用 6-TAMRA 紫外可见荧光强度的流式细胞术定量的摄取分布与使用 X 射线荧光显微镜测定的细胞铁含量的结果一致。
Organic-coated superparamagnetic iron oxide nanciparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using Xray fluorescence microscopy.