ABO3, a WRKY transcription factor, mediates plant responses to abscisic acid and drought tolerance in Arabidopsis.
ABO3, a WRKY transcription factor, mediates plant responses to abscisic acid and drought tolerance in Arabidopsis.
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ABO3 是一种 WRKY 转录因子，介导拟南芥植物对脱落酸的反应和耐旱性。
DOI：
10.1111/j.1365-313x.2010.04248.x
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发表时间：
2010-08
期刊：
影响因子：
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通讯作者：
Gong Z
中科院分区：
文献类型：
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作者：
Ren X;Chen Z;Liu Y;Zhang H;Zhang M;Liu Q;Hong X;Zhu JK;Gong Z
WRKY转录因子在植物中的生物学功能已得到广泛研究，但其在非生物胁迫中的作用仍不清楚。我们分离出一个阿坝过度敏感的突变体abo 3，它是由T-DNA插入At 1g 66600编码的WRKY转录因子AtWRKY 63破坏。该突变体在成苗和幼苗生长过程中均对阿坝高度敏感。然而，气孔关闭对阿坝的敏感性较低，并且abo 3突变体的耐旱性低于野生型。北方印迹分析表明，ABA反应性转录因子ABF 2/AREB 1在abo 3突变体中的表达显著低于野生型。abo 3突变还降低了胁迫诱导基因RD 29 A和COR 47的表达，尤其是在阿坝处理的早期。ABO 3在体外能够与ABF 2启动子中的W-box结合。 这些结果揭示了WRKY转录因子在植物响应阿坝和干旱胁迫中的重要作用。
The biological functions of WRKY transcription factors in plants have been widely studied, but their roles in abiotic stress are still not well understood. We isolated an ABA overly sensitive mutant, abo3, which is disrupted by a T-DNA insertion in At1g66600 encoding a WRKY transcription factor AtWRKY63. The mutant was hypersensitive to ABA in both seedling establishment and seedling growth. However, stomatal closure was less sensitive to ABA, and the abo3 mutant was less drought tolerant than the wild type. Northern blot analysis indicated that the expression of the ABA-responsive transcription factor ABF2/AREB1 was markedly lower in the abo3 mutant than in the wild type. The abo3 mutation also reduced the expression of stress-inducible genes RD29A and COR47, especially early during ABA treatment. ABO3 is able to bind the W-box in the promoter of ABF2in vitro. These results uncover an important role for a WRKY transcription factor in plant responses to ABA and drought stress.