Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle
Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle
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DOI：
10.1152/ajpregu.1999.276.6.r1682
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发表时间：
1999-06-01
影响因子：
2.8
通讯作者：
Jue, T
中科院分区：
文献类型：
--
作者：
Tran, TK;Sailasuta, N;Jue, T
H-1核磁共振检测到人腓肠肌的肌红蛋白(DeoxMb)和脱氧Hb(DeoxHb)的脱氧近端组氨酸NdeltaH信号。锻炼肌肉或压迫腿部以减少血流量会引起脱氧Mb信号的出现，随着细胞PO2的降低，信号的强度会增加。脱氧Mb信号以45-S的时间分辨率进行检测，并在加压袖带后5分钟内达到稳态水平。它的去饱和动力学与近红外光谱(NIRS)实验中观察到的一致，这意味着NIRS信号实际上是在监测Mb的去饱和。这一解释与β亚基脱氧Hb近端组氨酸NdeltaH信号的信号强度和去饱和度在73ppm是一致的。实验结果为观察骨骼肌中脱氧Mb和Hb信号的可行性和方法学奠定了基础，有助于阐明NIRS信号的来源，并为进一步研究骨骼肌中O2的调节奠定了基础。
H-1 NMR has detected both the deoxygenated proximal histidyl NdeltaH signals of myoglobin (deoxyMb) and deoxygenated Hb (deoxyHb) from human gastrocnemius muscle. Exercising the muscle or pressure cuffing the leg to reduce blood flow elicits the appearance of the deoxyMb signal, which increases in intensity as cellular PO2 decreases. The deoxyMb signal is detected with a 45-s time resolution and reaches a steady-state level within 5 min of pressure cuffing. Its desaturation kinetics match those observed in the near-infrared spectroscopy (NIRS) experiments, implying that the NIRS signals are actually monitoring Mb desaturation. That interpretation is consistent with the signal intensity and desaturation of the deoxyHb proximal histidyl NdeltaH signal from the beta-subunit at 73 parts per million. The experimental results establish the feasibility and methodology to observe the deoxyMb and Hb signals in skeletal muscle, help clarify the origin of the NIRS signal, and set a stage for continuing study of O-2 regulation in skeletal muscle.