2-Aminopurine Incorporation Perturbs the Dynamics and Structure of DNA
2-Aminopurine Incorporation Perturbs the Dynamics and Structure of DNA
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DOI：
10.1002/anie.201001312
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发表时间：
2010-01-01
影响因子：
16.6
通讯作者：
Ernsting, Nikolaus P.
中科院分区：
文献类型：
--
作者：
Dallmann, Andre;Dehmel, Lars;Ernsting, Nikolaus P.
2-氨基嘌呤（2AP）是腺嘌呤（A）的结构异构体，其中氨基位于C2而不是C6，并且可以与胸腺嘧啶（T）形成稳定的沃森-克里克（WC）型碱基对（图1）。[1，2]虽然天然核碱基根本不发射，但2AP显示出明显的荧光。更重要的是，其荧光量子产率在双链体形成后降低100倍。[3]许多研究利用2AP荧光来研究结构生物学和生物物理学中的问题：甲基转移酶诱导的碱基翻转，[4-6]锤头状核酶的构象变化和酶促切割，[7，8] T7 RNA聚合酶的启动子结合和清除，[9，10]引物的结合和链分离。
2-Aminopurine (2AP) is a structural isomer of adenine (A), in which the amino group is at C2 instead of C6, and can form stable Watson–Crick (WC) type base pairs with thymine (T)(Figure 1).[1, 2] While natural nucleobases do not emit at all, 2AP shows appreciable fluorescence. More importantly, its fluorescence quantum yield decreases 100-fold upon duplex formation.[3] Numerous studies exploit 2AP fluorescence to investigate problems in structural biology and biophysics: methyltransferase-induced base flipping,[4–6] conformational changes and enzymatic cleavage of the hammerhead ribozyme,[7, 8] promoter binding and clearance of T7 RNA polymerase,[9, 10] binding and strand separation of primer–