Intravenous eugenol causes hemorrhagic lung edema in rats : proposed oxidant mechanisms
Intravenous eugenol causes hemorrhagic lung edema in rats : proposed oxidant mechanisms
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发表时间：
1995-02
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通讯作者：
Wright Se;Baron Da;Heffner Je
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作者：
Wright Se;Baron Da;Heffner Je
虽然丁香酚，丁香油的活性酚成分，已被牵连作为非心源性肺水肿的原因，肺损伤的机制是未知的。我们研究了静脉滴注丁香酚对大鼠的影响，发现4 μ l和8 μ l丁香酚（6.52 mol/L）引起急性呼吸窘迫伴出血性肺水肿。组织学特征包括血管周围、间质和肺泡水肿，红细胞和中性粒细胞外渗至肺泡腔，肺泡毛细血管捕获中性粒细胞。此外，用丁香酚处理的肺具有增加的支气管肺泡灌洗液（BALF）蛋白质含量，并且在用8微升丁香酚处理的动物中肺湿干重比增加。静脉注射超氧化物歧化酶（SOD）或过氧化氢酶（而不是二甲基硫脲（DMTU））预处理后，4 μ L和8 μ L的丁香酚输注BALF蛋白质含量下降。SOD和过氧化氢酶，但不DMTU降低肺湿干重比的动物注入8微升丁香酚。我们的结论是，静脉注射丁香酚导致出血性肺水肿与肺内隔离的中性粒细胞，并建议，损伤可能至少部分氧化介导的。
Although eugenol, the active phenolic constituent of oil of cloves, has been implicated as a cause of noncardiogenic pulmonary edema, the mechanism of lung injury is unknown. We studied the effects of intravenous infusion of eugenol in rats and found that 4 microliters and 8 microliters of eugenol (6.52 mol/L) caused acute respiratory distress with hemorrhagic pulmonary edema. Histologic features included perivascular, interstitial, and alveolar edema with extravasation of red blood cells and neutrophils into the alveolar space and alveolar capillary trapping of neutrophils. In addition, lungs treated with eugenol had increased bronchoalveolar lavage fluid (BALF) protein content, and lung wet-to-dry weight ratios were increased in animals treated with 8 microliters eugenol. Pretreatment with intravenous superoxide dismutase (SOD) or catalase but not dimethylthiourea (DMTU) decreased BALF protein content after infusion of 4 microliters and 8 microliters of eugenol. SOD and catalase but not DMTU decreased lung wet-to-dry weight ratios in animals infused with 8 microliters of eugenol. We conclude that intravenous infusion of eugenol causes hemorrhagic pulmonary edema with intrapulmonary sequestration of neutrophils and suggest that the injury may be at least partly oxidant mediated.