Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1-dependent in cpr5
Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1-dependent in cpr5
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DOI：
10.1046/j.1365-313x.2001.2641041.x
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发表时间：
2001-05-01
期刊：
影响因子：
7.2
通讯作者：
Parker, JE
中科院分区：
文献类型：
--
作者：
Clarke, JD;Aarts, N;Parker, JE
拟南芥的系统获得性抗性（SAR）反应的特征在于水杨酸（SA）的积累、病程相关（PR）基因的表达以及对有毒细菌和卵菌病原体的抗性增强。cpr（PR基因的组成型表达子）突变体表达所有三种SAR表型。此外，cpr 5和cpr 6诱导与茉莉酸/乙烯介导的抗性途径的激活相关的防御相关基因PDF1.2的表达，cpr 5也形成自发性损伤。相比之下，eds 7（增强疾病易感性）突变消除了响应无毒病原体的抗性（R）基因产物的主要亚类所赋予的种族特异性抗性。EDS 7植物还表现出对毒性病原体的增加的易感性。上位性实验旨在探讨cpr和EDS 1介导的抗性途径之间的关系。我们发现eds 7的无效突变抑制了cpr 1和cpr 6的抗病表型。相比之下，EDS 1只部分抑制cpr 5的抗性，这使我们得出结论，cpr 5表达植物抗病性的EDS 1依赖和EDS 1独立成分。虽然eds 1不能阻止cpr 5叶片上的病斑形成，但它改变了它们的外观并减少了它们的传播。与cpr 5相比，这种表型差异与cpr 5eds 1植物的病原体定殖增加有关。这些数据使我们能够将EDS 1作为cpr 1和cpr 6介导的反应的必要下游组分，但表明EDS 1和cpr 5在植物防御中的关系更复杂。
The systemic acquired resistance (SAR) response in Arabidopsis is characterized by the accumulation of salicylic acid (SA), expression of the pathogenesis-related (PR) genes, and enhanced resistance to virulent bacterial and oomycete pathogens. The cpr (constitutive expressor of PR genes) mutants express all three SAR phenotypes. In addition, cpr5 and cpr6 induce expression of PDF1.2 a defense-related gene associated with activation of the jasmonate/ethylene-mediated resistance pathways, cpr5 also forms spontaneous lesions. In contrast, the eds7 (enhanced disease susceptibility) mutation abolishes race-specific resistance conferred by a major subclass of resistance (R) gene products in response to avirulent pathogens. eds7 plants also exhibit increased susceptibility to virulent pathogens. Epistasis experiments were designed to explore the relationship between the cpr- and EDS1-mediated resistance pathways. We found that a null eds7 mutation suppresses the disease resistance phenotypes of both cpr1 and cpr6. In contrast, eds1 only partially suppresses resistance in cpr5, leading us to conclude that cpr5 expresses both EDS1-dependent and EDS1-independent components of plant disease resistance. Although eds1 does not prevent lesion formation on cpr5 leaves, it alters their appearance and reduces their spread. This phenotypic difference is associated with increased pathogen colonization of cpr5 eds1 plants compared to cpr5. The data allow us to place EDS1 as a necessary downstream component of cpr1- and cpr6-mediated responses, but suggest a more complex relationship between EDS1 and cpr5 in plant defense.