Electrically charged GTAM membranes stimulate osteogenesis in rabbit calvarial defects.
Electrically charged GTAM membranes stimulate osteogenesis in rabbit calvarial defects.
复制标题
带电 GTAM 膜可刺激兔颅骨缺损的成骨作用。
DOI：
10.1034/j.1600-0501.1999.100508.x
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发表时间：
1999
影响因子：
4.3
通讯作者：
G. Cordioli
中科院分区：
文献类型：
--
作者：
A. Chierico;R. Valentini;Z. Majzoub;A. Piattelli;A. Scarano;L. Okun;G. Cordioli
在兔颅骨缺损模型中，使用中性、负电和正电的钛增强的GTAm膜，研究了膜保护的缺损区的骨新生。在36只兔的头盖骨上制造了两个标准的圆形8 mm宽和1 mm深的缺损区，内侧皮质完好无损。随后，用钛螺丝钉稳定的圆顶状钛强化GTAm膜覆盖缺损处，使膜的边缘接近骨表面。实验动物分为6组，每组6只，分别于5天(组1)、10天(组2)、3周(组3)、5周(组4)、10周(组5)、20周(组6)处死。72个膜按电荷分布，每组产生4个带正电的、4个带负电的和4个中性的穹顶。组织形态计量学分析显示，带负电荷的穹顶有更快和更多的骨新生。在10天时，负电荷膜部位的平均新生骨面积为27.95%，而在相同的评估时间间隔内，中性和带正电圆顶的新骨形成可以忽略不计。随着时间的推移，带负电的膜比中性膜支持更多的新骨形成，而带正电的膜显示的新骨最少。这项工作证明，负向电刺激可以加速和维持新骨生成。这些结果也暗示了带负电荷的GTAm膜在临床上的潜在应用。
Bone neogenesis was studied in membrane-protected defects in a rabbit calvaria defect model using neutral, negatively, and positively charged titanium-reinforced GTAM membranes. Two standardized circular 8 mm wide and 1 mm deep defects were created in the calvaria of 36 rabbits leaving the inner cortex intact. The defects were subsequently covered with dome-shaped Ti-reinforced GTAM membranes stabilized with a titanium screw allowing the edges of the membranes to be closely approximated to the bone surface. The animals were divided into 6 groups of 6 rabbits each and were sacrificed at 5 days (Group 1), 10 days (Group 2), 3 weeks (Group 3), 5 weeks (Group 4), 10 weeks (Group 5), and 20 weeks (Group 6). The distribution of the 72 membranes according to charge yielded 4 positively charged, 4 negatively charged and 4 neutral domes in each group. Histomorphometric analysis showed a more rapid and increased bone neogenesis with the negatively charged domes. A mean total area of 27.95% of newly-formed bone was observed in the negatively charged membrane sites at 10 days while negligible bone formation occurred with the neutral and positively charged domes at the same evaluation interval. Over time, negatively charged membranes supported more new-bone formation than neutral membranes while positively charged membranes showed the least new bone. This work demonstrates that negative electrical stimulation accelerates and maintains bone neogenesis. These results also suggest the potential applications of negatively charged GTAM membranes in clinical settings.