Gene targeting in filamentous fungi: the benefits of impaired repair
Gene targeting in filamentous fungi: the benefits of impaired repair
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DOI：
10.1016/j.fbr.2007.02.004
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发表时间：
2007-02-01
影响因子：
6
通讯作者：
Krappmann, Sven
中科院分区：
文献类型：
--
作者：
Krappmann, Sven
已发表的真菌基因组序列的范围要求复杂的遗传工具来解决注释的基因产物的功能和特征。基因打靶是评估给定蛋白质的细胞作用的首选方法，试图用选择性标记替换编码序列或将其融合到功能模块，例如。G.荧光标记。这种方法依赖于外源DNA片段的同源整合，其速率由双链断裂修复的两个竞争过程决定：非法重组和同源重组。通过抑制前一个过程，靶向真菌基因的进展最近已经实现;对于几种丝状真菌，当这种所谓的非同源末端连接途径被阻断时，同源整合率的显著增加是显而易见的，这打开了高通量综合功能研究的前景。(c)2007年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
The extent of published fungal genome sequences call for sophisticated genetic tools to address the functions and features of annotated gene products. Gene targeting is the method of choice to assess the cellular role of a given protein, trying to replace the coding sequence by selectable markers or to fuse it to functional modules, e. g. fluorescent labels. This approach relies on homologous integration of exogenous DNA fragments, the rate of which is determined by two competing processes of double strand break repair: illegitimate and homologous recombination. Advances in targeting fungal genes have recently been achieved by suppression of the former process; for several filamentous fungi a dramatic increase in homologous integration rates is evident when this so-called non-homologous end joining pathway is inoperative, which opens the prospect of comprehensive functional studies at high throughput. (c) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.