Psychrophilicity of Bacillus psychrosaccharolyticus: A proteomic study
Psychrophilicity of Bacillus psychrosaccharolyticus: A proteomic study
复制标题
解糖芽孢杆菌的嗜冷性：蛋白质组学研究
作者：
Jong;Hye Sook Kim;Gyoo Yeol Jung;M. Nam;J. Chung;J. Kim;J. Yoo;Chan;Ohoak Kwon
采用蛋白质组学方法对革兰氏阳性菌嗜冷性进行了研究。由于缺乏革兰氏阳性菌的基因组序列，用电喷雾电离-四极杆飞行时间串联质谱仪分析了131个蛋白质点，并使用了一个未发表的翻译重叠群数据库和来自NCBI的非冗余革兰氏阳性细菌蛋白质数据库进行了鉴定。对冷应激反应蛋白质组学行为的研究结果表明，应激反应对代谢功能和保护机制的整体上调可能是嗜冷性的主要原因。
Psychrophilicity of Gram‐positive bacterium, Bacillus psychrosaccharolyticus was investigated in a proteomic approach. One hundred and thirty‐one protein spots were analyzed by electrospray ionization‐quadrupole‐time of flight‐tandem mass spectrometry and identified using an unpublished translated contig database as well as a nonredundant Gram‐positive bacteria protein database from NCBI because of the lack of a genome sequence of this organism. Results focused on proteomic behavior of cold‐response show that global up‐regulation of metabolic functions and protective mechanism by stress responses might play a major role in psychrophilicity of B. psychrosaccharolyticus.