Effect of Tempol on the prevention of irradiation-induced mucositis in miniature pigs.
Effect of Tempol on the prevention of irradiation-induced mucositis in miniature pigs.
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DOI：
10.1111/odi.12667
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发表时间：
2017-09
期刊：
影响因子：
3.8
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Hu L;Wang Y;Cotrim AP;Zhu Z;Gao R;Zheng C;Goldsmith CM;Jin L;Zhang C;Mitchell JB;Baum BJ;Wang S
本研究的目的是（i）建立一个有用的小型猪（迷你猪）模型，用于辐射诱导的口腔粘膜炎和（ii）评价Tempol预防其发展的效果。用6戈伊连续5天照射小型猪的整个口腔。为了防止辐射损伤，在照射前10分钟用30 mg kg−1 Tempol处理小型猪（n = 4），而单独照射组同样注射生理盐水（n = 4）。每3天使用口腔粘膜炎评分和目视检查对病变进行分级，并在第18天进行多个部位的活检。每3天测量一次体重、胸围和腹围。第一次照射后约12天开始出现病变，照射后约30天愈合。在第一次照射后第18天计算舌和颊粘膜的上皮厚度。使用这种治疗策略，Tempol在照射后提供了适度的溃疡保护。这项研究建立了一个有用的大型动物模型，辐射诱导的口腔粘膜炎，并显示适度的有益影响Tempol在限制组织损伤。后一项发现可能对预防接受头颈部癌症放射治疗的患者的口腔粘膜炎有潜在价值。
The goals of this study were to (i) establish a useful miniature pig (minipig) model for irradiation-induced oral mucositis and (ii) evaluate the effect of Tempol to prevent its development. Minipigs were irradiated with 6 Gy for five consecutive days targeting the entire oral cavity. To prevent radiation damage, minipigs were treated with 30 mg kg−1 Tempol 10 min before irradiation (n = 4), while the radiation-alone group was similarly injected with saline (n = 4). Lesions were graded using an oral mucositis score and visual inspection every 3 days, and biopsy of multiple sites was performed at day 18. Weight and chest and abdominal circumferences were measured every 3 days. Lesions began about 12 days after the first irradiation fraction and healed about 30 days after irradiation. Epithelial thickness was calculated on the lingual and buccal mucosa on the 18th day after the first irradiation fraction. Tempol provided modest protection from ulceration after irradiation using this treatment strategy. This study established a useful large animal model for irradiation-induced oral mucositis and showed modest beneficial effects of Tempol in limiting tissue damage. The latter finding may be potentially valuable in preventing oral mucositis in patients receiving irradiation for head and neck cancers.
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影响因子：
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