The use of cis-parinaric acid to determine lipid peroxidation in human erythrocyte membranes. Comparison of normal and sickle erythrocyte membranes.
The use of cis-parinaric acid to determine lipid peroxidation in human erythrocyte membranes. Comparison of normal and sickle erythrocyte membranes.
复制标题
使用顺式石蜡酸测定人红细胞膜中的脂质过氧化。
DOI：
10.1016/0005-2736(88)90313-6
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
OpdenKamp,JA
中科院分区：
文献类型：
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作者：
VandenBerg,JJ;Kuypers,FA;Qju,JH;Chiu,D;Lubin,B;Roelofsen,B;OpdenKamp,JA
最近发展起来的Parinaric acid分析方法为研究生物膜系统中的过氧化过程提供了可能性。以人红细胞膜为模型，考察了几种引发体系，以及残留血红蛋白在鬼膜制剂中的作用。自由基发生系统的有效性似乎强烈依赖于自由基是在膜水平还是在水相中产生。因此，浓度为1.0-1.5 mM的过氧化异丙苯与亚微摩尔水平的氯化血红素-Fe3+作为膜结合辅因子，被发现是一种非常有效的过氧化引发剂。与过氧化异丙苯相结合，膜结合的血红蛋白在促进过氧化方面似乎比水相中的血红蛋白有效约6倍。结果比较正常和镰刀状红细胞膜的过氧化反应表明，镰状红细胞膜上氧化应激的增加可能是由于镰状血红蛋白与膜的结合增强，但部分原因是镰状血红蛋白具有更强的促进过氧化的能力。从实验中可以得出的促进过氧化能力的顺序是：血红素&>镰状血红蛋白&>正常血红蛋白。
The recently developed parinaric acid assay is shown to offer possibilities for studying peroxidation processes in biological membrane systems. Taking the human erythrocyte membrane as a model, several initiating systems were investigated, as well as the effect of residual hemoglobin in ghost membrane preparations. The effectivity of a radical generating system appeared to be strongly dependent upon whether radicals are generated at the membrane level or in the water phase. Thus, cumene hydroperoxide at concentrations of 1.0–1.5 mM was found to be a very efficient initiator of peroxidation in combination with submicromolar levels of hemin-Fe3+as membrane-bound cofactor. In combination with cumene hydroperoxide, membrane-bound hemoglobin appeared to be about 6-times more effective in promoting peroxidation than hemoglobin in the water phase. Results comparing the behaviour of normal and sickle erythrocyte ghost suspensions in the peroxidation assay suggest that the increased oxidative stress on sickle erythrocyte membranes could be due to enhanced membrane binding of sickle hemoglobin, but also partly to a characteristically higher capability of sickle hemoglobin to promote peroxidation. The order of peroxidation-promoting capabilities that could be derived from the experiments was hemin > sickle hemoglobin > normal hemoglobin.