Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated
Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated
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DOI：
10.1046/j.1365-313x.2001.01124.x
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发表时间：
2001-09-01
期刊：
影响因子：
7.2
通讯作者：
Peterson, T
中科院分区：
文献类型：
--
作者：
Cocciolone, SM;Chopra, S;Peterson, T
玉米p1基因编码红色素生物合成的Myb同源调节因子。为了研究p1基因的组织特异性调节，用组合两个等位基因的启动子和cDNA序列的构建体转化玉米植物;这两个等位基因的色素沉着模式不同：P1-wr（白色果皮/红穗轴）和P1-rr（红色果皮/红穗轴）。令人惊讶的是，所有启动子/cDNA组合产生的转基因植物具有红色果皮和红色穗轴（RR模式），表明P1-wr启动子和编码的蛋白质可以在果皮中起作用。一些RR模式的转基因植物产生具有白色果皮和红色穗轴的后代植物（WR模式），并且这种组织特异性的转换与转基因甲基化增加相关。果皮色素沉着和DNA甲基化之间的一个类似的负相关性，观察到某些自然的P1等位基因，它具有标准的P1-WR等位基因的基因结构特征，但赋予红色果皮色素沉着，并始终低于标准的P1-WR等位基因的甲基化。虽然我们不能排除可能存在的组织特异性调控元件内的P1非编码序列或侧翼区，从转基因和天然等位基因的数据表明，组织特异性色素沉着模式的P1-WR表型的特征是表观遗传控制。
The maize p1 gene encodes a Myb-homologous regulator of red pigment biosynthesis. To investigate the tissue-specific regulation of the p1 gene, maize plants were transformed with constructs combining promoter and cDNA sequences of two alleles; which differ in pigmentation patterns: P1-wr (white pericarp/red cob) and P1-rr (red pericarp/red cob). Surprisingly, all promoter/cDNA combinations produced transgenic plants with red pericarp and red cob (RR pattern), indicating that the P1-wr promoter and encoded protein can function in pericarp. Some of the RR patterned transgenic plants produced progeny plants with white pericarp and red cob (WR pattern), and this switch in tissue-specificity correlated with increased transgene methylation. A similar inverse correlation between pericarp pigmentation and DNA methylation was observed for certain natural p1 alleles, which have a gene structure characteristic of standard P1-wr alleles, but which confer red pericarp pigmentation and are consistently less methylated than standard P1-wr alleles. Although we cannot rule out the possible existence of tissue-specific regulatory elements within the p1 non-coding sequences or flanking regions, the data from transgenic and natural alleles suggest that the tissue-specific pigmentation pattern characteristic of the P1-wr phenotype is epigenetically controlled.